Electrophoretic separation of human embryonic globin demonstrates "α-thalassemia" in human leukemia cell line K562

Blanche P. Alter, Sabra C. Goff

Research output: Contribution to journalArticlepeer-review

Abstract

Human embryonic, fetal, and adult globin chains (ζ, ε, Aγ, Gγ, β, α) can be separated by electrophoresis on Triton Acid urea gels. K562, a human leukemia cell line, was induced with hemin, labelled with [3H]-leucine, and globin synthesis analyzed. All globins except β were produced. ε > ζ; Gγ:Aγ=70:30; non-α:α=>2:1. Thus, hemin-induced K562 synthesized embryonic and fetal globin chains, and had globin synthetic imbalance, with "α-thalassemia.".

Original languageEnglish (US)
Pages (from-to)843-848
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume94
Issue number3
DOIs
StatePublished - Jun 16 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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