Electron paramagnetic resonance measurement of the distance between the metal binding sites of transferrin

J. L. Zweier

Research output: Contribution to journalArticlepeer-review

Abstract

The distance between the two metal binding sites of human serum transferrin is calculated from measurements made of the paramagnetic broadening of the EPR spectrum of Cu2+ bound at the NH2-terminal (B) site from Fe3+ bound at the COOH-terminal (A) site. Complexes of monoferric(A)-monocupric(B) transferrin, monocupric(B) transferrin, and monogallium(A)-monocupric(B) transferrin were prepared under identical conditions. Computer simulation and direct measurement of the EPR spectra from these complexes demonstrate a paramagnetic broadening of 0.6 ± 0.1 G in the line width of the Cu2+ spectrum due to the paramagnetic Fe3+ bound at the other site. The distance between the two metal binding sites is calculated to be 41.6 ± 2.8 Å.

Original languageEnglish (US)
Pages (from-to)13759-13760
Number of pages2
JournalJournal of Biological Chemistry
Volume258
Issue number22
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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