Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr

Robert M. Weis, Teruhisa Hirai, Anas Chalah, Martin Kessel, Peter J. Peters, Sriram Subramaniam

Research output: Contribution to journalArticle

Abstract

The serine receptor (Tsr) from Escherichia coli is representative of a large family of transmembrane receptor proteins that mediate bacterial chemotaxis by influencing cell motility through signal transduction pathways. Tsr and other chemotaxis receptors form patches in the inner membrane that are often localized at the poles of the bacteria. In an effort to understand the structural constraints that dictate the packing of receptors in the plane of the membrane, we have used electron microscopy to examine ordered assemblies of Tsr in membrane extracts isolated from cells engineered to overproduce the receptor. Three types of assemblies were observed: ring-like "micelles" with a radial arrangement of receptor subunits, two-dimensional crystalline arrays with approximate hexagonal symmetry, and "zippers," which are receptor bilayers that result from the antiparallel interdigitation of cytoplasmic domains. The registration among Tsr molecules in the micelle and zipper assemblies was sufficient for identification of the receptor domains and for determination of their contributions to the total receptor length. The overall result of this analysis is compatible with an atomic model of the receptor dimer that was constructed primarily from the X-ray crystal structures of the periplasmic and cytoplasmic domains. Significantly, the micelle and zipper structures were also observed in fixed, cryosectioned cells expressing the Tsr receptor at high abundance, suggesting that the modes of Tsr assembly found in vitro are relevant to the situation in the cell.

Original languageEnglish (US)
Pages (from-to)3636-3643
Number of pages8
JournalJournal of Bacteriology
Volume185
Issue number12
DOIs
StatePublished - Jun 2003
Externally publishedYes

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Chemotaxis
Electrons
Membranes
Micelles
Cytoplasmic Structures
Bacterial Proteins
Cell Extracts
Cell Movement
serine receptor
Signal Transduction
Electron Microscopy
X-Rays
Escherichia coli
Bacteria

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr. / Weis, Robert M.; Hirai, Teruhisa; Chalah, Anas; Kessel, Martin; Peters, Peter J.; Subramaniam, Sriram.

In: Journal of Bacteriology, Vol. 185, No. 12, 06.2003, p. 3636-3643.

Research output: Contribution to journalArticle

Weis, RM, Hirai, T, Chalah, A, Kessel, M, Peters, PJ & Subramaniam, S 2003, 'Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr', Journal of Bacteriology, vol. 185, no. 12, pp. 3636-3643. https://doi.org/10.1128/JB.185.12.3636-3643.2003
Weis, Robert M. ; Hirai, Teruhisa ; Chalah, Anas ; Kessel, Martin ; Peters, Peter J. ; Subramaniam, Sriram. / Electron microscopic analysis of membrane assemblies formed by the bacterial chemotaxis receptor Tsr. In: Journal of Bacteriology. 2003 ; Vol. 185, No. 12. pp. 3636-3643.
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