Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin

Sriram Subramaniam, Mark Gerstein, Dieter Oesterhelt, Richard Henderson

Research output: Contribution to journalArticle

Abstract

Structural changes are central to the mechanism of light-driven proton transport by bacteriorhodopsin, a seven-helix membrane protein. The main intermediate formed upon light absorption is M, which occurs between the proton release and uptake steps of the photocycle. To investigate the structure of the M intermediate, we have carried out electron diffraction studies with two-dimensional crystals of wild-type bacteriorhodopsin and the Asp96 → Gly mutant. The M intermediate was trapped by rapidly freezing the crystals in liquid ethane following illumination with a xenon flash lamp at 5 and 25°C. Here, we present 3.5 Å resolution Fourier projection maps of the differences between the M intermediate and the ground state of bacteriorhodopsin. The most prominent structural changes are observed in the vicinity of helices F and G and are localized to the cytoplasmic half of the membrane.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalThe EMBO journal
Volume12
Issue number1
StatePublished - 1993

Fingerprint

Bacteriorhodopsins
Electron diffraction
Electrons
Protons
Liquid Crystals
Crystals
Xenon
Ethane
Lighting
Electric lamps
Freezing
Light absorption
Ground state
Membrane Proteins
Cell Membrane
Membranes
Light
Liquids

Keywords

  • Conformational change
  • M-intermediate
  • Retinal proteins
  • Seven-helix receptors
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Subramaniam, S., Gerstein, M., Oesterhelt, D., & Henderson, R. (1993). Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin. The EMBO journal, 12(1), 1-8.

Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin. / Subramaniam, Sriram; Gerstein, Mark; Oesterhelt, Dieter; Henderson, Richard.

In: The EMBO journal, Vol. 12, No. 1, 1993, p. 1-8.

Research output: Contribution to journalArticle

Subramaniam, S, Gerstein, M, Oesterhelt, D & Henderson, R 1993, 'Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin', The EMBO journal, vol. 12, no. 1, pp. 1-8.
Subramaniam S, Gerstein M, Oesterhelt D, Henderson R. Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin. The EMBO journal. 1993;12(1):1-8.
Subramaniam, Sriram ; Gerstein, Mark ; Oesterhelt, Dieter ; Henderson, Richard. / Electron diffraction analysis of structural changes in the photocycle of bacteriorhodopsin. In: The EMBO journal. 1993 ; Vol. 12, No. 1. pp. 1-8.
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