Electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis

Karel Šlais, Miroslava Štastná

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, we suggest new electrolyte system for fast preparative electrofocusing in wide pH range. It is based on bidirectional ITP with multiple counterions and spacers created by commercially available defined simple buffers. The migration course of proposed focusing model can be simulated in advance by using separation conditions and electrolyte components that are consequently applied during the experiments. The suggested electrolyte system allows high current densities at the initial stages of focusing without danger of local overheating, which strongly reduces the time needed for analysis completion. The performance of the electrolyte system is demonstrated by the focusing of synthetic colored low molecular weight indicators and proteins in the arrangements with both linear narrow strip and nonwoven fabric sheet with continuous flow.

Original languageEnglish (US)
Pages (from-to)2438-2445
Number of pages8
JournalElectrophoresis
Volume35
Issue number17
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • Bidirectional isotachophoresis
  • Electrofocusing
  • Electrolyte system
  • Moving boundary electrophoresis
  • Wide pH range

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Medicine(all)

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