Electrocardiographic characterization of cardiac hypertrophy in mice that overexpress the ErbB2 receptor tyrosine kinase

Polina Sysa-Shah, Lars L. Sørensen, M. Roselle Abraham, Kathleen L Gabrielson

Research output: Contribution to journalArticle

Abstract

Electrocardiography is an important method for evaluation and risk stratification of patients with cardiac hypertrophy. We hypothesized that the recently developed transgenic mouse model of cardiac hypertrophy (ErbB2tg) will display distinct ECG features, enabling WT (wild type) mice to be distinguished from transgenic mice without using conventional PCR genotyping. We evaluated more than 2000 mice and developed specific criteria for genotype determination by using cageside ECG, during which unanesthetized mice were manually restrained for less than 1 min. Compared with those from WT counterparts, the ECG recordings of ErbB2tg mice were characterized by higher P- and R-wave amplitudes, broader QRS complexes, inverted T waves, and ST interval depression. Pearson's correlation matrix analysis of combined WT and ErbB2tg data revealed significant correlation between heart weight and the ECG parameters of QT interval (corrected for heart rate), QRS interval, ST height, R amplitude, P amplitude, and PR interval. In addition, the left ventricular posterior wall thickness as determined by echocardiography correlated with ECG-determined ST height, R amplitude, QRS interval; echocardiographic left ventricular mass correlated with ECG-determined ST height and PR interval. In summary, we have determined phenotypic ECG criteria to differentiate ErbB2tg from WT genotypes in 98.8% of mice. This inexpensive and time-efficient ECG-based phenotypic method might be applied to differentiate between genotypes in other rodent models of cardiac hypertrophy. Furthermore, with appropriate modifications, this method might be translated for use in other species.

Original languageEnglish (US)
Pages (from-to)295-307
Number of pages13
JournalComparative Medicine
Volume65
Issue number4
StatePublished - Aug 1 2015

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Cardiomegaly
Receptor Protein-Tyrosine Kinases
Electrocardiography
hypertrophy
tyrosine
phosphotransferases (kinases)
receptors
mice
genotype
Genotype
genetically modified organisms
Transgenic Mice
electrocardiography
echocardiography
genotyping
Echocardiography
heart rate
rodents
animal models
methodology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Electrocardiographic characterization of cardiac hypertrophy in mice that overexpress the ErbB2 receptor tyrosine kinase. / Sysa-Shah, Polina; Sørensen, Lars L.; Roselle Abraham, M.; Gabrielson, Kathleen L.

In: Comparative Medicine, Vol. 65, No. 4, 01.08.2015, p. 295-307.

Research output: Contribution to journalArticle

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