Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon

Vesselin Nikolov, Janice Lin, Mikhail Merzlyakov, Kalina A Hristova, Peter C. Searson

Research output: Contribution to journalArticle

Abstract

Bilayer membranes on solid supports are used for fundamental studies of biophysical properties and for the development of biosensors and other devices. Here we report on electrically addressable bilayer membranes formed by Langmuir-Blodgett (LB)-based deposition on single-crystal silicon. The incorporation of a polymer cushion ensures high lipid mobility in both the lower and upper leaflet, allowing the potential for combined investigations of electrical, structural, and dynamic characteristics of membrane-associated proteins. Impedance spectroscopy is used to demonstrate that the lipid bilayers are robust and reproducible with an impedance of about 104 Ω cm2 and a capacitance of about 0.8 μF cm-2. The ability to characterize ion channels is demonstrated using the model system gramicidin. These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components.

Original languageEnglish (US)
Pages (from-to)13040-13045
Number of pages6
JournalLangmuir
Volume23
Issue number26
DOIs
StatePublished - Dec 18 2007

Fingerprint

Silicon
electrical measurement
Single crystals
membranes
Membranes
single crystals
silicon
Gramicidin
lipids
Lipid bilayers
impedance
cushions
Ion Channels
Biosensors
Polymers
Membrane Proteins
Capacitance
bioinstrumentation
Spectroscopy
dynamic characteristics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon. / Nikolov, Vesselin; Lin, Janice; Merzlyakov, Mikhail; Hristova, Kalina A; Searson, Peter C.

In: Langmuir, Vol. 23, No. 26, 18.12.2007, p. 13040-13045.

Research output: Contribution to journalArticle

Nikolov, Vesselin ; Lin, Janice ; Merzlyakov, Mikhail ; Hristova, Kalina A ; Searson, Peter C. / Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon. In: Langmuir. 2007 ; Vol. 23, No. 26. pp. 13040-13045.
@article{cc3c97208a7646d3b8040627b61a3da1,
title = "Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon",
abstract = "Bilayer membranes on solid supports are used for fundamental studies of biophysical properties and for the development of biosensors and other devices. Here we report on electrically addressable bilayer membranes formed by Langmuir-Blodgett (LB)-based deposition on single-crystal silicon. The incorporation of a polymer cushion ensures high lipid mobility in both the lower and upper leaflet, allowing the potential for combined investigations of electrical, structural, and dynamic characteristics of membrane-associated proteins. Impedance spectroscopy is used to demonstrate that the lipid bilayers are robust and reproducible with an impedance of about 104 Ω cm2 and a capacitance of about 0.8 μF cm-2. The ability to characterize ion channels is demonstrated using the model system gramicidin. These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components.",
author = "Vesselin Nikolov and Janice Lin and Mikhail Merzlyakov and Hristova, {Kalina A} and Searson, {Peter C.}",
year = "2007",
month = "12",
day = "18",
doi = "10.1021/la702147m",
language = "English (US)",
volume = "23",
pages = "13040--13045",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "26",

}

TY - JOUR

T1 - Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon

AU - Nikolov, Vesselin

AU - Lin, Janice

AU - Merzlyakov, Mikhail

AU - Hristova, Kalina A

AU - Searson, Peter C.

PY - 2007/12/18

Y1 - 2007/12/18

N2 - Bilayer membranes on solid supports are used for fundamental studies of biophysical properties and for the development of biosensors and other devices. Here we report on electrically addressable bilayer membranes formed by Langmuir-Blodgett (LB)-based deposition on single-crystal silicon. The incorporation of a polymer cushion ensures high lipid mobility in both the lower and upper leaflet, allowing the potential for combined investigations of electrical, structural, and dynamic characteristics of membrane-associated proteins. Impedance spectroscopy is used to demonstrate that the lipid bilayers are robust and reproducible with an impedance of about 104 Ω cm2 and a capacitance of about 0.8 μF cm-2. The ability to characterize ion channels is demonstrated using the model system gramicidin. These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components.

AB - Bilayer membranes on solid supports are used for fundamental studies of biophysical properties and for the development of biosensors and other devices. Here we report on electrically addressable bilayer membranes formed by Langmuir-Blodgett (LB)-based deposition on single-crystal silicon. The incorporation of a polymer cushion ensures high lipid mobility in both the lower and upper leaflet, allowing the potential for combined investigations of electrical, structural, and dynamic characteristics of membrane-associated proteins. Impedance spectroscopy is used to demonstrate that the lipid bilayers are robust and reproducible with an impedance of about 104 Ω cm2 and a capacitance of about 0.8 μF cm-2. The ability to characterize ion channels is demonstrated using the model system gramicidin. These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components.

UR - http://www.scopus.com/inward/record.url?scp=38049138241&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38049138241&partnerID=8YFLogxK

U2 - 10.1021/la702147m

DO - 10.1021/la702147m

M3 - Article

C2 - 18004893

AN - SCOPUS:38049138241

VL - 23

SP - 13040

EP - 13045

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 26

ER -