Electrical measurements of bilayer membranes formed by Langmuir-Blodgett deposition on single-crystal silicon

Vesselin Nikolov, Janice Lin, Mikhail Merzlyakov, Kalina Hristova, Peter C. Searson

Research output: Contribution to journalArticle

Abstract

Bilayer membranes on solid supports are used for fundamental studies of biophysical properties and for the development of biosensors and other devices. Here we report on electrically addressable bilayer membranes formed by Langmuir-Blodgett (LB)-based deposition on single-crystal silicon. The incorporation of a polymer cushion ensures high lipid mobility in both the lower and upper leaflet, allowing the potential for combined investigations of electrical, structural, and dynamic characteristics of membrane-associated proteins. Impedance spectroscopy is used to demonstrate that the lipid bilayers are robust and reproducible with an impedance of about 104 Ω cm2 and a capacitance of about 0.8 μF cm-2. The ability to characterize ion channels is demonstrated using the model system gramicidin. These results demonstrate that artificial bilayers formed by LB deposition have many unique advantages for electrical measurements of membranes and their components.

Original languageEnglish (US)
Pages (from-to)13040-13045
Number of pages6
JournalLangmuir
Volume23
Issue number26
DOIs
StatePublished - Dec 18 2007

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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