Electric charge stoichiometry of calcium translocation in mitochondria

Baltazar Reynafarje, Albert L. Lehninger

Research output: Contribution to journalArticlepeer-review

Abstract

The electric charge stoichiometry of calcium ion transport into respiring mitochondria has been measured, using H+- and Ca2+-sensitive electrodes to determine the number of H+ ions ejected in electroneutral exchange for Ca. In three types of system the ratio H+ ejected/Ca2+ taken up was very close to 2.0 when phosphate movements are prevented. Thus Ca is transported into respiring mitochondria in an electrogenic process in which each Ca carries 2 positive charges. The conclusion of Moyle and Mitchell (FEBS Lett. 73, 131-136, 1977) that Ca carries only one (+) charge is shown to be based on inadequate data and assumptions.

Original languageEnglish (US)
Pages (from-to)1273-1279
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume77
Issue number4
DOIs
StatePublished - Aug 22 1977

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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