TY - JOUR
T1 - Electric charge stoichiometry of calcium translocation in mitochondria
AU - Reynafarje, Baltazar
AU - Lehninger, Albert L.
PY - 1977/8/22
Y1 - 1977/8/22
N2 - The electric charge stoichiometry of calcium ion transport into respiring mitochondria has been measured, using H+- and Ca2+-sensitive electrodes to determine the number of H+ ions ejected in electroneutral exchange for Ca. In three types of system the ratio H+ ejected/Ca2+ taken up was very close to 2.0 when phosphate movements are prevented. Thus Ca is transported into respiring mitochondria in an electrogenic process in which each Ca carries 2 positive charges. The conclusion of Moyle and Mitchell (FEBS Lett. 73, 131-136, 1977) that Ca carries only one (+) charge is shown to be based on inadequate data and assumptions.
AB - The electric charge stoichiometry of calcium ion transport into respiring mitochondria has been measured, using H+- and Ca2+-sensitive electrodes to determine the number of H+ ions ejected in electroneutral exchange for Ca. In three types of system the ratio H+ ejected/Ca2+ taken up was very close to 2.0 when phosphate movements are prevented. Thus Ca is transported into respiring mitochondria in an electrogenic process in which each Ca carries 2 positive charges. The conclusion of Moyle and Mitchell (FEBS Lett. 73, 131-136, 1977) that Ca carries only one (+) charge is shown to be based on inadequate data and assumptions.
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U2 - 10.1016/S0006-291X(77)80117-4
DO - 10.1016/S0006-291X(77)80117-4
M3 - Article
C2 - 20098
AN - SCOPUS:0017700304
SN - 0006-291X
VL - 77
SP - 1273
EP - 1279
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -