TY - JOUR
T1 - Elastic liposome-mediated transdermal immunization enhanced the immunogenicity of P. falciparum surface antigen, MSP-119
AU - Tyagi, Rajeev K.
AU - Garg, Neeraj K.
AU - Jadon, Rajesh
AU - Sahu, Tejram
AU - Katare, Om Prakash
AU - Dalai, Sarat K.
AU - Awasthi, Amit
AU - Marepally, Srujan K.
N1 - Funding Information:
The authors are grateful for the fellowship (SRF) and grant provided by the Council of Scientific and Industrial Research (CSIR HRDG) New Delhi, India. Rajeev Tyagi is thankful to Dr. V.S. Chauhan (ICGEB, New Delhi) for providing gift sample of antigen PfMSP-1 19 . The electron microscopy facility provided by AIIMS, New Delhi is duly solicited and acknowledged.
Funding Information:
Funding: This work was funded by a grant from Department of Biotechnology (DBT), New Delhi, India and Council of Scientific and Industrial Research (CSIR HRDG) New Delhi, India. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study.
Publisher Copyright:
© 2015 Elsevier Ltd.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2015/8/26
Y1 - 2015/8/26
N2 - Transdermal immunization results in poor immunogenicity, which can be attributed to poor permeability of antigens through the skin. Therefore, elastic liposome, ultradeformable lipid vesicles, may overcome the challenges faced during transdermal immunization. This versatile carrier proves better vehicle for transcutaneous delivery of protein, peptide and nucleic acid antigens. The present results are suggestive of improved immunogenicity of carboxyl-terminal 19kDa fragment of merozoite surface protein-1 (PfMSP-119) of Plasmodium falciparum when administered subcutaneously through elastic liposomes. The prepared elastic liposomes were characterized with respect to vesicles shape and surface morphology, size and size distribution, entrapment efficiency, elasticity, stability and in vitro release. Humoral and cell-mediated immune (CMI) response elicited by topically applied PfMSP-119-loaded elastic liposomes, intramuscularly administered alum-adsorbed PfMSP-119 solution, and topically applied PfMSP-119-loaded conventional liposomes were compared and normalized with vehicle control. Results suggest greater transcutaneous immunization via elastic liposomes, and induced robust and perdurable IgG-specific antibody and cytophilic isotype responses. We report to have achieved sizeable CMI activating factor (IFNγ), a crucial player in conferring resistance to asexual blood stage malaria, responses with elastic liposomes when compared with other formulations. The fluorescence microscopy and histopathology results are suggestive of prominent skin permeation and biodistribution, and demonstrate efficient delivery of malaria antigen via elastic liposomes to immunocompetent Langerhans cells (LC) and lymphatics. In conclusion, elastic liposomal formulation provided greater entrapment efficiency, enhanced penetration and heightened and long-lasting immune response. Moreover, effective immunoadjuvant property of this carrier justifies its potential for improved vaccine delivery, and opens new avenues to explore further on the development of malaria vaccine.
AB - Transdermal immunization results in poor immunogenicity, which can be attributed to poor permeability of antigens through the skin. Therefore, elastic liposome, ultradeformable lipid vesicles, may overcome the challenges faced during transdermal immunization. This versatile carrier proves better vehicle for transcutaneous delivery of protein, peptide and nucleic acid antigens. The present results are suggestive of improved immunogenicity of carboxyl-terminal 19kDa fragment of merozoite surface protein-1 (PfMSP-119) of Plasmodium falciparum when administered subcutaneously through elastic liposomes. The prepared elastic liposomes were characterized with respect to vesicles shape and surface morphology, size and size distribution, entrapment efficiency, elasticity, stability and in vitro release. Humoral and cell-mediated immune (CMI) response elicited by topically applied PfMSP-119-loaded elastic liposomes, intramuscularly administered alum-adsorbed PfMSP-119 solution, and topically applied PfMSP-119-loaded conventional liposomes were compared and normalized with vehicle control. Results suggest greater transcutaneous immunization via elastic liposomes, and induced robust and perdurable IgG-specific antibody and cytophilic isotype responses. We report to have achieved sizeable CMI activating factor (IFNγ), a crucial player in conferring resistance to asexual blood stage malaria, responses with elastic liposomes when compared with other formulations. The fluorescence microscopy and histopathology results are suggestive of prominent skin permeation and biodistribution, and demonstrate efficient delivery of malaria antigen via elastic liposomes to immunocompetent Langerhans cells (LC) and lymphatics. In conclusion, elastic liposomal formulation provided greater entrapment efficiency, enhanced penetration and heightened and long-lasting immune response. Moreover, effective immunoadjuvant property of this carrier justifies its potential for improved vaccine delivery, and opens new avenues to explore further on the development of malaria vaccine.
KW - Cellular and humoral immunity
KW - Elastic liposome
KW - Immunization
KW - Langerhans cells
KW - MSP-119
KW - Transdermal delivery
KW - Vaccine
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U2 - 10.1016/j.vaccine.2015.06.054
DO - 10.1016/j.vaccine.2015.06.054
M3 - Article
C2 - 26141014
AN - SCOPUS:84939490021
VL - 33
SP - 4630
EP - 4638
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 36
ER -