Ehrlichia and spotted fever group rickettsiae surveillance in Amblyomma americanum in Virginia through use of a novel six-plex real-time PCR assay

David N. Gaines, Darwin J. Operario, Suzanne Stroup, Ellen Stromdahl, Chelsea Wright, Holly Gaff, James Broyhill, Joshua Smith, Douglas Norris, Tyler Henning, Agape Lucas, Eric Houpt

Research output: Contribution to journalArticle

Abstract

The population of the lone star tick Amblyomma americanum has expanded in North America over the last several decades. It is known to be an aggressive and nondiscriminatory biter and is by far the most common human-biting tick encountered in Virginia. Few studies of human pathogen prevalence in ticks have been conducted in our state since the mid-twentieth century. We developed a six-plex real-time PCR assay to detect three Ehrlichia species (E. chaffeensis, E. ewingii, and Panola Mountain Ehrlichia) and three spotted fever group Rickettsiae (SFGR; R. amblyommii, R. parkeri, and R. rickettsii) and used it to test A. americanum from around the state. Our studies revealed a presence of all three Ehrlichia species (0-24.5%) and a high prevalence (50-80%) of R. amblyommii, a presumptively nonpathogenic SFGR, in all regions surveyed. R. parkeri, previously only detected in Virginia's Amblyomma maculatum ticks, was found in A. americanum in several surveyed areas within two regions having established A. maculatum populations. R. rickettsii was not found in any sample tested. Our study provides the first state-wide screening of A. americanum ticks in recent history and indicates that human exposure to R. amblyommii and to Ehrlichiae may be common. The high prevalence of R. amblyommii, serological cross-reactivity of all SFGR members, and the apparent rarity of R. rickettsii in human biting ticks across the eastern United States suggest that clinical cases of tick-borne disease, including ehrlichiosis, may be commonly misdiagnosed as Rocky Mountain spotted fever, and that suspicion of other SFGR as well as Ehrlichia should be increased. These data may be of relevance to other regions where A. americanum is prevalent.

Original languageEnglish (US)
Pages (from-to)307-316
Number of pages10
JournalVector-Borne and Zoonotic Diseases
Volume14
Issue number5
DOIs
StatePublished - May 1 2014

Fingerprint

Ehrlichia
Rickettsia
Ticks
Real-Time Polymerase Chain Reaction
Fever
Rocky Mountain Spotted Fever
Ehrlichiosis
Tick-Borne Diseases
North America
Diagnostic Errors
Population
History

Keywords

  • Ehrlichia
  • PCR
  • Real
  • Rickettsia
  • Ticks
  • time RT
  • Vector borne

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Virology
  • Medicine(all)

Cite this

Ehrlichia and spotted fever group rickettsiae surveillance in Amblyomma americanum in Virginia through use of a novel six-plex real-time PCR assay. / Gaines, David N.; Operario, Darwin J.; Stroup, Suzanne; Stromdahl, Ellen; Wright, Chelsea; Gaff, Holly; Broyhill, James; Smith, Joshua; Norris, Douglas; Henning, Tyler; Lucas, Agape; Houpt, Eric.

In: Vector-Borne and Zoonotic Diseases, Vol. 14, No. 5, 01.05.2014, p. 307-316.

Research output: Contribution to journalArticle

Gaines, DN, Operario, DJ, Stroup, S, Stromdahl, E, Wright, C, Gaff, H, Broyhill, J, Smith, J, Norris, D, Henning, T, Lucas, A & Houpt, E 2014, 'Ehrlichia and spotted fever group rickettsiae surveillance in Amblyomma americanum in Virginia through use of a novel six-plex real-time PCR assay', Vector-Borne and Zoonotic Diseases, vol. 14, no. 5, pp. 307-316. https://doi.org/10.1089/vbz.2013.1509
Gaines, David N. ; Operario, Darwin J. ; Stroup, Suzanne ; Stromdahl, Ellen ; Wright, Chelsea ; Gaff, Holly ; Broyhill, James ; Smith, Joshua ; Norris, Douglas ; Henning, Tyler ; Lucas, Agape ; Houpt, Eric. / Ehrlichia and spotted fever group rickettsiae surveillance in Amblyomma americanum in Virginia through use of a novel six-plex real-time PCR assay. In: Vector-Borne and Zoonotic Diseases. 2014 ; Vol. 14, No. 5. pp. 307-316.
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