EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics

Daniel M. Freed, Nicholas J. Bessman, Anatoly Kiyatkin, Emanuel Salazar-Cavazos, Patrick O. Byrne, Jason O. Moore, Christopher C. Valley, Kathryn M. Ferguson, Daniel J. Leahy, Diane S. Lidke, Mark A. Lemmon

Research output: Contribution to journalArticlepeer-review

Abstract

Epidermal growth factor receptor (EGFR) regulates many crucial cellular programs, with seven different activating ligands shaping cell signaling in distinct ways. Using crystallography and other approaches, we show how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. As a consequence, EREG or EPGN induce less stable EGFR dimers than EGF—making them partial agonists of EGFR dimerization. Unexpectedly, this weakened dimerization elicits more sustained EGFR signaling than seen with EGF, provoking responses in breast cancer cells associated with differentiation rather than proliferation. Our results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. Our results also suggest parallels between partial and/or biased agonism in RTKs and G-protein-coupled receptors, as well as new therapeutic opportunities for correcting RTK signaling output. Receptor tyrosine kinases operate under principles of biased agonism to shape signaling outputs.

Original languageEnglish (US)
Pages (from-to)683-695.e18
JournalCell
Volume171
Issue number3
DOIs
StatePublished - Oct 19 2017

Keywords

  • biased agonist
  • cell fate decision
  • crystallography
  • dimerization
  • growth factor
  • kinetic proofreading
  • negative feedback
  • phosphatase
  • receptor tyrosine kinase
  • signaling specificity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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