Efficient Transformation of the Medicinal Mushroom Ganoderma lucidum

Li Sun, Huaqing Cai, Weihong Xu, Yuanlei Hu, Yin Gao, Zhongping Lin

Research output: Contribution to journalArticle

Abstract

Ganoderma lucidum is a well-known and important medicinal mushroom, but its genetic modification has not been reported. We developed an efficient procedure for isolation and regeneration of protoplasts from G. lucidum. To construct a vector for high-level expression of heterologous genes in G. lucidum, the 1.4-kb regulatory region of the glyceraldehyde-3-phosphate dehydrogenase gene (GPD) was isolated from the genomic DNA of Lentinus edodes, and the GPD promoter was fused to the β-glucuronidase (GUS) and bialaphos resistance (bar) genes. Using the resulting construct, p301-bG1, an efficient transformation system based on electroporation was established for G. lucidum. GUS expression was observed among transformants conferring bialaphos resistance, indicating that the L. edodes GPD promoter can be used for expression of exogenous genes in G. lucidum. We also studied green fluorescent protein (GFP) as another reporter for transformation of G. lucidum. The L. edodes GPD promoter was fused respectively to the GFP and bar genes, and the resulting construct, p301-bg, was introduced into G. lucidum. Stable GFP expression in transformants was detectable by fluorescence microscopy, suggesting the suitability of GFP as a reporter system in transformation of this mushroom. This is the first report of an efficient transformation system for G. lucidum using different reporters, paving the way for genetic modification of this famous medicinal mushroom.

Original languageEnglish (US)
JournalPlant Molecular Biology Reporter
Volume19
Issue number4
StatePublished - 2001
Externally publishedYes

Fingerprint

Reishi
Ganoderma lucidum
Agaricales
Genes
Glyceraldehyde-3-Phosphate Dehydrogenases
glyceraldehyde-3-phosphate dehydrogenase
Shiitake Mushrooms
Green Fluorescent Proteins
green fluorescent protein
Lentinula edodes
genes
Glucuronidase
promoter regions
genetic engineering
Gene Expression
Electroporation
electroporation
medicinal fungi
Protoplasts
Nucleic Acid Regulatory Sequences

Keywords

  • Bialaphos resistance
  • Ganoderma lucidum
  • GFP
  • GPD promoter
  • GUS
  • Lentinus edodes

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Sun, L., Cai, H., Xu, W., Hu, Y., Gao, Y., & Lin, Z. (2001). Efficient Transformation of the Medicinal Mushroom Ganoderma lucidum. Plant Molecular Biology Reporter, 19(4).

Efficient Transformation of the Medicinal Mushroom Ganoderma lucidum. / Sun, Li; Cai, Huaqing; Xu, Weihong; Hu, Yuanlei; Gao, Yin; Lin, Zhongping.

In: Plant Molecular Biology Reporter, Vol. 19, No. 4, 2001.

Research output: Contribution to journalArticle

Sun, L, Cai, H, Xu, W, Hu, Y, Gao, Y & Lin, Z 2001, 'Efficient Transformation of the Medicinal Mushroom Ganoderma lucidum', Plant Molecular Biology Reporter, vol. 19, no. 4.
Sun, Li ; Cai, Huaqing ; Xu, Weihong ; Hu, Yuanlei ; Gao, Yin ; Lin, Zhongping. / Efficient Transformation of the Medicinal Mushroom Ganoderma lucidum. In: Plant Molecular Biology Reporter. 2001 ; Vol. 19, No. 4.
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