Efficient protein boosting after plasmid DNA or recombinant adenovirus immunization with HIV-1 vaccine constructs

Yuuei Shu, Sarah Winfrey, Zhi yong Yang, Ling Xu, Srinivas S. Rao, Indresh Srivastava, Susan W. Barnett, Gary J. Nabel, John R. Mascola

Research output: Contribution to journalArticlepeer-review

Abstract

DNA plasmids and recombinant adenovirus serotype-5 (rAd5) vectors are being studied in human clinical trials as HIV-1 vaccine candidates. Each elicits robust T-cell responses and modest antibody levels. Since protein immunization alone elicits antibody but not CD8 T-cell responses, we studied protein boosting of DNA and rAd5 HIV-1 vaccine vectors. A single Env protein immunization provided a marked boost in antibody titer in guinea pigs primed with either DNA or rAd5 vaccines, and the resulting antibody binding and neutralization levels were similar to those attained after thee sequential protein immunizations. Since both T-cell immunity and neutralizing antibodies are thought to be required for protection against HIV-1, it may be possible to establish a balanced T-cell and antibody response with appropriate vectored vaccines and improve the neutralizing antibody titer with protein boosting.

Original languageEnglish (US)
Pages (from-to)1398-1408
Number of pages11
JournalVaccine
Volume25
Issue number8
DOIs
StatePublished - Feb 9 2007

Keywords

  • Adjuvant
  • HIV
  • Neutralizing antibodies

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Efficient protein boosting after plasmid DNA or recombinant adenovirus immunization with HIV-1 vaccine constructs'. Together they form a unique fingerprint.

Cite this