Efficient generation of recombinant adenoviral vectors by Cre-lox recombination in vitro

Kazunori Aoki, Christopher Barker, Xavier Danthinne, Michael J. Imperiale, Gary J. Nabel

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Although recombinant adenovirus vectors are attractive for use in gene expression studies and therapeutic applications, the construction of these vectors remains relatively time-consuming. We report here a strategy that simplifies the production of adenoviruses using the Cre-loxP system. Materials and Methods: Full-length recombinant adenovirus DNA was generated in vitro by Cre-mediated recombination between loxP sites in a linearized shuttle plasmid containing a transgene and adenovirus genomic DNA. Results: After transfection of Cre-treated DNA into 293 cells, replication-defective viral vectors were rapidly obtained without detectable wild-type virus. Conclusion: This system facilitates the development of recombinant adenoviral vectors for basic and clinical research.

Original languageEnglish (US)
Pages (from-to)224-231
Number of pages8
JournalMolecular Medicine
Volume5
Issue number4
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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