Efficient generation of fully reprogrammed human iPS cells via polycistronic retroviral vector and a new cocktail of chemical compounds

Zhonghui Zhang, Yongxing Gao, Albert Gordon, Zack Z. Wang, Zhijian Qian, Wen Shu Wu

Research output: Contribution to journalArticle

Abstract

Direct reprogramming of human somatic cells into induced pluripotent stem (iPS) cells by defined transcription factors (TFs) provides great potential for regenerative medicine and biomedical research. This procedure has many challenges, including low reprogramming efficiency, many partially reprogrammed colonies, somatic coding mutations in the genome, etc. Here, we describe a simple approach for generating fully reprogrammed human iPS cells by using a single polycistronic retroviral vector expressing four human TFs in a single open reading frame (ORF), combined with a cocktail containing three small molecules (Sodium butyrate, SB431542, and PD0325901). Our results demonstrate that human iPS cells generated by this approach express human ES cells markers and exhibit pluripotency demonstrated by their abilities to differentiate into the three germ layers in vitro and in vivo. Notably, this approach not only provides a much faster reprogramming process but also significantly diminishes partially reprogrammed iPS cell colonies, thus facilitating efficient isolation of desired fully reprogrammed iPS cell colonies.

Original languageEnglish (US)
Article numbere26592
JournalPLoS One
Volume6
Issue number10
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

retroviral vectors
Induced Pluripotent Stem Cells
Chemical compounds
chemical compounds
Stem cells
Transcription Factors
transcription factors
Germ Layers
Regenerative Medicine
Butyric Acid
biomedical research
somatic cells
Open Reading Frames
open reading frames
Biomedical Research
medicine
Genes
induced pluripotent stem cells
Genome
mutation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Efficient generation of fully reprogrammed human iPS cells via polycistronic retroviral vector and a new cocktail of chemical compounds. / Zhang, Zhonghui; Gao, Yongxing; Gordon, Albert; Wang, Zack Z.; Qian, Zhijian; Wu, Wen Shu.

In: PLoS One, Vol. 6, No. 10, e26592, 2011.

Research output: Contribution to journalArticle

Zhang, Zhonghui ; Gao, Yongxing ; Gordon, Albert ; Wang, Zack Z. ; Qian, Zhijian ; Wu, Wen Shu. / Efficient generation of fully reprogrammed human iPS cells via polycistronic retroviral vector and a new cocktail of chemical compounds. In: PLoS One. 2011 ; Vol. 6, No. 10.
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