Efficient development of Plasmodium liver stage-specific memory CD8 + T cells during the course of blood-stage malarial infection

Julius C.R. Hafalla, Urvashi Rai, Dabeiba Bernal-Rubio, Ana Rodriguez, Fidel Zavala

Research output: Contribution to journalArticlepeer-review

Abstract

Immunity to Plasmodium liver stages in individuals in malaria-endemic areas is inextricably linked to concomitant blood-stage parasitemia. Although Plasmodium sporozoite infection induces measurable CD8+ T cell responses, the development of memory T cells during active erythrocytic infection remains uncharacterized. Using transgenic T cells, we assessed antigen-specific effector CD8+ T cell responses induced by normal (NorSpz) and radiation-attenuated (IrrSpz) Plasmodium yoelii sporozoites. The magnitude, phenotypic activation, and differentiation pathway of CD8+ T cells were similarly induced by NorSpz and IrrSpz. Moreover, in normal mice, memory T cells elicited after priming with NorSpz and IrrSpz generated identical recall responses after a heterologous boost strategy. Furthermore, these recall responses exhibited comparable in vivo antiparasite activity. Our results indicate that sporozoites that retain their infective capacity induce memory CD8+ T cells that are robustly recalled by secondary immunization. Thus, erythrocytic infection does not preclude the establishment ofmemory CD8+ T cell responses to malarial liver stages.

Original languageEnglish (US)
Pages (from-to)1827-1835
Number of pages9
JournalJournal of Infectious Diseases
Volume196
Issue number12
DOIs
StatePublished - Dec 15 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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