TY - JOUR
T1 - Efficient development of Plasmodium liver stage-specific memory CD8 + T cells during the course of blood-stage malarial infection
AU - Hafalla, Julius C.R.
AU - Rai, Urvashi
AU - Bernal-Rubio, Dabeiba
AU - Rodriguez, Ana
AU - Zavala, Fidel
N1 - Funding Information:
Financial support: National Institutes of Health (grant AI044375 to F.Z. and grant AI053698 to A.R.); American Liver Foundation (Gene Varian Research Fellowship to J.C.R.H.). J.C.R.H. was awarded a Gates Foundation travel grant to present the results of this work at the 2005 Gordon Research Conference on Malaria and is currently an Incoming USA/Canada Fellow of the Royal Society (UK).
PY - 2007/12/15
Y1 - 2007/12/15
N2 - Immunity to Plasmodium liver stages in individuals in malaria-endemic areas is inextricably linked to concomitant blood-stage parasitemia. Although Plasmodium sporozoite infection induces measurable CD8+ T cell responses, the development of memory T cells during active erythrocytic infection remains uncharacterized. Using transgenic T cells, we assessed antigen-specific effector CD8+ T cell responses induced by normal (NorSpz) and radiation-attenuated (IrrSpz) Plasmodium yoelii sporozoites. The magnitude, phenotypic activation, and differentiation pathway of CD8+ T cells were similarly induced by NorSpz and IrrSpz. Moreover, in normal mice, memory T cells elicited after priming with NorSpz and IrrSpz generated identical recall responses after a heterologous boost strategy. Furthermore, these recall responses exhibited comparable in vivo antiparasite activity. Our results indicate that sporozoites that retain their infective capacity induce memory CD8+ T cells that are robustly recalled by secondary immunization. Thus, erythrocytic infection does not preclude the establishment ofmemory CD8+ T cell responses to malarial liver stages.
AB - Immunity to Plasmodium liver stages in individuals in malaria-endemic areas is inextricably linked to concomitant blood-stage parasitemia. Although Plasmodium sporozoite infection induces measurable CD8+ T cell responses, the development of memory T cells during active erythrocytic infection remains uncharacterized. Using transgenic T cells, we assessed antigen-specific effector CD8+ T cell responses induced by normal (NorSpz) and radiation-attenuated (IrrSpz) Plasmodium yoelii sporozoites. The magnitude, phenotypic activation, and differentiation pathway of CD8+ T cells were similarly induced by NorSpz and IrrSpz. Moreover, in normal mice, memory T cells elicited after priming with NorSpz and IrrSpz generated identical recall responses after a heterologous boost strategy. Furthermore, these recall responses exhibited comparable in vivo antiparasite activity. Our results indicate that sporozoites that retain their infective capacity induce memory CD8+ T cells that are robustly recalled by secondary immunization. Thus, erythrocytic infection does not preclude the establishment ofmemory CD8+ T cell responses to malarial liver stages.
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U2 - 10.1086/522965
DO - 10.1086/522965
M3 - Article
C2 - 18190264
AN - SCOPUS:39149095964
SN - 0022-1899
VL - 196
SP - 1827
EP - 1835
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 12
ER -