Efficient delivery of DNA vaccines using human papillomavirus pseudovirions

S. Peng, A. Monie, T. H. Kang, C. F. Hung, R. Roden, T. C. Wu

Research output: Contribution to journalArticle

Abstract

We have examined non-replicative human papillomavirus (HPV) pseudovirions as an approach in the delivery of naked DNA vaccines without safety concerns associated with live viral vectors. In this study, we have generated HPV-16 pseudovirions encapsidating a DNA vaccine encoding the model antigen, ovalbumin (OVA) (HPV16-OVA pseudovirions). Vaccination with HPV16-OVA pseudovirions subcutaneously elicited significantly stronger OVA-specific CD8 T-cell immune responses compared with OVA DNA vaccination via gene gun in a dose-dependent manner. We showed that a single amino acid mutation in the L2 minor capsid protein that eliminates the infectivity of HPV16-OVA pseudovirion significantly decreased the antigen-specific CD8 T-cell responses in vaccinated mice. Furthermore, a subset of CD11c cells and B220 cells in draining lymph nodes became labeled on vaccination with fluorescein isothiocyanate-labeled HPV16-OVA pseudovirions in injected mice. HPV pseudovirions were found to infect bone marrow-derived dendritic cells (BMDCs) in vitro. We also showed that pretreatment of HPV16-GFP pseudovirions with furin leads to enhanced HPV16-OVA pseudovirion infection of BMDCs and OVA antigen presentation. Our data suggest that DNA vaccines delivered using HPV pseudovirions represent an efficient delivery system that can potentially affect the field of DNA vaccine delivery.

Original languageEnglish (US)
Pages (from-to)1453-1464
Number of pages12
JournalGene Therapy
Volume17
Issue number12
DOIs
StatePublished - Dec 1 2010

Keywords

  • human papillomavirus
  • pseudovirions
  • vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Efficient delivery of DNA vaccines using human papillomavirus pseudovirions'. Together they form a unique fingerprint.

  • Cite this