Efficacy of three different methods for side port incision wound sealing

Fabiana K. Kashiwabuchi, Yasin A. Khan, Murilo W. Rodrigues, Jiangxia Wang, Peter J McDonnell, Yassine Daoud

Research output: Contribution to journalArticle

Abstract

To evaluate wound leakage and bacteria-sized particle influx in differently corneal sealed side port incisions. Methods: Four 1.5mm tunnel squared incisions were created in each of four cadaveric human eyes. In each cornea, one incision was left unsealed, whereas the other three incisions were sealed using a 10-0 nylon suture, cyanoacrylate glue, or stromal hydration, respectively. A Seidel and an India ink test were performed on each eye. During each Seidel test, flourescein was applied, the IOP increased from 15 to 80mmHg, and the IOP at which each incision started to leak recorded. During each India ink test, ink was placed on the eye and rinsed out with balanced salt solution (BSS). Ink penetration was then measured by planimetry at physiologic conditions and after an IOP plunge from 80mmHg to 0mmHg. Results: Regardless of IOP variations, no leakage or ink inflow was observed through the glued incisions. In contrast, leakage did occur in the other three sealing methods, albeit at significantly different IOP levels in each one (p=0.013). Ink inflow occurred in these sealing methods at physiologic IOP and, to a significantly greater extent, after the IOP challenge (p

Original languageEnglish (US)
Pages (from-to)379-382
Number of pages4
JournalRevista Brasileira de Oftalmologia
Volume72
Issue number6
DOIs
StatePublished - 2013

Fingerprint

Ink
Wounds and Injuries
Cyanoacrylates
Nylons
Adhesives
Cornea
Sutures
Salts
Bacteria
chinese ink

Keywords

  • Biomechanics
  • Cataract extraction
  • Endophthalmitis
  • Intraocular pressure
  • Limbus corneae

ASJC Scopus subject areas

  • Ophthalmology
  • Surgery

Cite this

Efficacy of three different methods for side port incision wound sealing. / Kashiwabuchi, Fabiana K.; Khan, Yasin A.; Rodrigues, Murilo W.; Wang, Jiangxia; McDonnell, Peter J; Daoud, Yassine.

In: Revista Brasileira de Oftalmologia, Vol. 72, No. 6, 2013, p. 379-382.

Research output: Contribution to journalArticle

Kashiwabuchi, Fabiana K. ; Khan, Yasin A. ; Rodrigues, Murilo W. ; Wang, Jiangxia ; McDonnell, Peter J ; Daoud, Yassine. / Efficacy of three different methods for side port incision wound sealing. In: Revista Brasileira de Oftalmologia. 2013 ; Vol. 72, No. 6. pp. 379-382.
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