Efficacy of pig-to-rhesus lamellar corneal xenotransplantation

Hyuk Jin Choi, Mee Kum Kim, Hyun Ju Lee, Jung Hwa Ko, So Hee Jeong, Jae Il Lee, Byoung Chol Oh, Hee Jung Kang, Won Ryang Wee

Research output: Contribution to journalArticle

Abstract

PURPOSE. To solve the shortage of donor corneas, a decellularizing method based on hypertonic saline treatment was introduced, and a favorable outcome was observed in pig-to-rabbit lamellar corneal transplantation. This study was an investigation of the efficacy of pig-to-nonhuman primate lamellar corneal transplantation, using both decellularized and fresh porcine corneas to assess feasibility as a substitute for human corneas. METHODS. Nine Chinese rhesus macaques underwent lamellar corneal transplantation using both decellularized (n = 5) and fresh (n = 4) porcine corneas. Clinically acceptable graft size (7.5 mm in diameter) and minimal immunosuppression based on topical and systemic corticosteroids were applied. Rejection signs, histology of porcine grafts, and serial changes in recipients' blood profile, including memory T-cell subset, anti-αGal and donor pig-specific antibodies, and complement were evaluated. Changes in aqueous complement concentration were also assessed at 4 weeks after transplantation. RESULTS. Of the decellularized porcine lamellar grafts, 80% remained transparent for more than 6 months, whereas half of the fresh porcine lamellar grafts developed chronic rejection. Rejected grafts showed extensive cellular infiltration, predominantly CD8 + T lymphocytes and macrophages. Immunologic profiles of the recipients with rejected grafts showed a significant increase in the concentration of aqueous complement, an enhancement of memory T cells, and an abrupt increase in donor pig-specific antibodies. CONCLUSIONS. The findings suggested that decellularized porcine cornea could be a promising substitute for human corneal allograft. Fresh porcine cornea may be a feasible option for a substitute if combined with more potent immunosuppression or if obtained from transgenic pigs with complement-regulatory proteins.

Original languageEnglish (US)
Pages (from-to)6643-6650
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number9
DOIs
StatePublished - Aug 1 2011

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Choi, H. J., Kim, M. K., Lee, H. J., Ko, J. H., Jeong, S. H., Lee, J. I., Oh, B. C., Kang, H. J., & Wee, W. R. (2011). Efficacy of pig-to-rhesus lamellar corneal xenotransplantation. Investigative Ophthalmology and Visual Science, 52(9), 6643-6650. https://doi.org/10.1167/iovs.11-7273