Efficacy of immune therapy in early experimental Naegleria fowleri meningitis

G. J. Lallinger, S. L. Reiner, David W Cooke, D. L. Toffaletti, J. R. Perfect, D. L. Granger, D. T. Durack

Research output: Contribution to journalArticle

Abstract

Naegleria fowleri meningoencephalitis is usually fatal in humans despite treatment. As a new approach, we tested intracisternal passive immune therapy in rabbits with amebic meningoencephalitis by using antinaegleria immune serum, an immunoglobulin G fraction, and a newly developed monoclonal antibody to N. fowleri. Both the immune serum and an immunoglobulin G fraction isolated from it by affinity chromatography provided a consistent, although temporary, protective effect, shown by prolongation of survival (P = 0.001). Multiple doses of immune serum further prolonged survival (P = 0.005). The protective effect of serum was retained after heating to 56°C. We then developed a monoclonal antibody to N. fowleri which provided similar protection. Passive intracisternal antibody therapy might serve as an adjunctive component in the treatment of amebic meningoencephalitis.

Original languageEnglish (US)
Pages (from-to)1289-1293
Number of pages5
JournalInfection and Immunity
Volume55
Issue number5
StatePublished - 1987
Externally publishedYes

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Naegleria fowleri
Central Nervous System Protozoal Infections
Meningitis
Immune Sera
Immunoglobulin G
Monoclonal Antibodies
Meningoencephalitis
Survival
Affinity Chromatography
Heating
Therapeutics
Rabbits
Antibodies
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Lallinger, G. J., Reiner, S. L., Cooke, D. W., Toffaletti, D. L., Perfect, J. R., Granger, D. L., & Durack, D. T. (1987). Efficacy of immune therapy in early experimental Naegleria fowleri meningitis. Infection and Immunity, 55(5), 1289-1293.

Efficacy of immune therapy in early experimental Naegleria fowleri meningitis. / Lallinger, G. J.; Reiner, S. L.; Cooke, David W; Toffaletti, D. L.; Perfect, J. R.; Granger, D. L.; Durack, D. T.

In: Infection and Immunity, Vol. 55, No. 5, 1987, p. 1289-1293.

Research output: Contribution to journalArticle

Lallinger, GJ, Reiner, SL, Cooke, DW, Toffaletti, DL, Perfect, JR, Granger, DL & Durack, DT 1987, 'Efficacy of immune therapy in early experimental Naegleria fowleri meningitis', Infection and Immunity, vol. 55, no. 5, pp. 1289-1293.
Lallinger GJ, Reiner SL, Cooke DW, Toffaletti DL, Perfect JR, Granger DL et al. Efficacy of immune therapy in early experimental Naegleria fowleri meningitis. Infection and Immunity. 1987;55(5):1289-1293.
Lallinger, G. J. ; Reiner, S. L. ; Cooke, David W ; Toffaletti, D. L. ; Perfect, J. R. ; Granger, D. L. ; Durack, D. T. / Efficacy of immune therapy in early experimental Naegleria fowleri meningitis. In: Infection and Immunity. 1987 ; Vol. 55, No. 5. pp. 1289-1293.
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