Effects of Zeng Sheng Ping/ACAPHA on malignant brain tumor growth and notch signaling

Kah Jing Lim, Kda Rajan, Charles G. Eberhart

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Aim: Zeng Sheng Ping (ZSP) is a traditional herbal remedy used to prevent progression and growth of neoplastic lesions. It has been shown to inhibit Notch2 expression in a murine lung cancer model, leading us to investigate its therapeutic potential in Notch-dependent brain tumors. Materials and Methods: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)-2H-tetrazolium (MTS), apoptosis, and quantitative real-time polymerase chain reaction (RT-PCR) analyses were performed in glioma and medulloblastoma cell lines, and morphological analyses in DAOY flank xenografts. Results: ZSP inhibited brain tumor growth in vitro, in part, by apoptotic induction. Down-regulation of the Notch2 receptor, the pathway target Hairy/Enhancer of Split homolog 1 (Hes1), and of the stem cell markers Nestin and CD133 was also observed. Reductions in tumor mass and increases in the necrotic fraction of DAOY xenografts, in mice treated with oral ZSP were also observed, but these were not significant. Conclusion: ZSP can block brain tumor growth and the expression of Notch pathway members and stem cell markers in vitro.

Original languageEnglish (US)
Pages (from-to)2689-2696
Number of pages8
JournalAnticancer research
Volume32
Issue number7
StatePublished - Jul 2012

Keywords

  • ACAPHA
  • Antitumor-B
  • DAOY medulloblastoma cells
  • Glioblastoma
  • Medulloblastoma
  • Notch
  • U87 glioblastoma cells
  • Zeng Sheng Ping

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Effects of Zeng Sheng Ping/ACAPHA on malignant brain tumor growth and notch signaling'. Together they form a unique fingerprint.

Cite this