Effects of ventilation on the collection of exhaled breath in humans

Keary A. Cope, Michael T. Watson, W. Michael Foster, Shelley S. Sehnert, Terence H Risby

Research output: Contribution to journalArticle

Abstract

A computerized system has been developed to monitor tidal volume, respiration rate, mouth pressure, and carbon dioxide during breath collection. This system was used to investigate variability in the production of breath biomarkers over an 8-h period. Hyperventilation occurred when breath was collected from spontaneously breathing study subjects (n = 8). Therefore, breath samples were collected from study subjects whose breathing were paced at a respiration rate of 10 breaths/min and whose tidal volumes were gauged according to body mass. In this "paced breathing" group (n = 16), end-tidal concentrations of isoprene and ethane correlated with end-tidal carbon dioxide levels [Spearman's rank correlation test (rs) = 0.64, P = 0.008 and rs = 0.50, P = 0.05, respectively]. Ethane also correlated with heart rate (rs = 0.52, P <0.05). There was an inverse correlation between transcutaneous pulse oximetry and exhaled carbon monoxide (rs = -0.64, P = 0.008). Significant differences were identified between men (n = 8) and women (n = 8) in the concentrations of carbon monoxide (4 parts per million in men vs. 3 parts per million in women; P = 0.01) and volatile sulfur-containing compounds (134 parts per billion in men vs. 95 parts per billion in women; P = 0.016). There was a peak in ethanol concentration directly after food consumption and a significant decrease in ethanol concentration 2 h later (P = 0.01; n = 16). Sulfur-containing molecules increased linearly throughout the study period (β = 7.4, P <0.003). Ventilation patterns strongly influence quantification of volatile analytes in exhaled breath and thus, accordingly, the breathing pattern should be controlled to ensure representative analyses.

Original languageEnglish (US)
Pages (from-to)1371-1379
Number of pages9
JournalJournal of Applied Physiology
Volume96
Issue number4
DOIs
StatePublished - Apr 2004

Fingerprint

Ventilation
Respiration
Ethane
Tidal Volume
Carbon Monoxide
Respiratory Rate
Carbon Dioxide
Ethanol
Transcutaneous Blood Gas Monitoring
Sulfur Compounds
Oximetry
Hyperventilation
Sulfur
Mouth
Biomarkers
Heart Rate
Pressure
Food

Keywords

  • Biomarker
  • Breath analytes
  • Ventilation pattern

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of ventilation on the collection of exhaled breath in humans. / Cope, Keary A.; Watson, Michael T.; Foster, W. Michael; Sehnert, Shelley S.; Risby, Terence H.

In: Journal of Applied Physiology, Vol. 96, No. 4, 04.2004, p. 1371-1379.

Research output: Contribution to journalArticle

Cope, Keary A. ; Watson, Michael T. ; Foster, W. Michael ; Sehnert, Shelley S. ; Risby, Terence H. / Effects of ventilation on the collection of exhaled breath in humans. In: Journal of Applied Physiology. 2004 ; Vol. 96, No. 4. pp. 1371-1379.
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