TY - JOUR
T1 - Effects of vaccination with 10-valent pneumococcal non-typeable Haemophilus influenza protein D conjugate vaccine (PHiDCV) on the nasopharyngeal microbiome of Kenyan toddlers
AU - Feazel, Leah M.
AU - Santorico, Stephanie A.
AU - Robertson, Charles E.
AU - Bashraheil, Mahfudh
AU - Scott, J. Anthony G.
AU - Frank, Daniel N.
AU - Hammitt, Laura L.
N1 - Funding Information:
The authors of this manuscript have the following competing interests: Leah M. Faezel, Stephanie A. Santorico, Charles E. Robertson, Mahfudh Bashraheil declare no conflict of interest in publication of this study. J. Anthony G. Scott has received research funding from GlaxoSmithKline Biologicals and support for travel/accommodation at a meeting sponsored by Merck. Daniel N. Frank has received research funding from Pfizer Inc., and Laura L. Hammitt has received research funding from GlaxoSmithKline Biologicals and Pfizer Inc, and has participated in a Scientific Input Engagement For Merck. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.
Publisher Copyright:
© 2015 Feazel et al.
PY - 2015/6/17
Y1 - 2015/6/17
N2 - Objective: Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). Methods: Profiles of the nasopharyngeal microbiota of 60 children aged 12-59 months, who had been randomized to receive 2 doses of PHiD-CV (n=30) or Hepatitis A vaccine (n=30) 60 days apart, were constructed by 16S rRNA gene pyrosequencing of swab specimens collected before vaccination and 180 days after dose 1. Results: Prior to vaccination, Moraxella catarrhalis (median of 12.3%of sequences/subject), Streptococcus pneumoniae (4.4%) and Corynebacterium spp. (5.6%) were the most abundant nasopharyngeal bacterial species. Vaccination with PHiD-CV did not significantly alter the species composition, abundance, or prevalence of known pathogens. Distinct microbiomes were identified based on the abundances of Streptococcus, Moraxella, and Haemophilus species. These microbiomes shifted in composition over the study period and were independent of age, sex, school attendance, antibiotic exposure, and vaccination. Conclusions: Vaccination of children with two doses of PHiD-CV did not significantly alter the nasopharyngeal microbiome. This suggests limited replacement carriage with pathogens other than non-vaccine strains of S. pneumoniae.
AB - Objective: Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). Methods: Profiles of the nasopharyngeal microbiota of 60 children aged 12-59 months, who had been randomized to receive 2 doses of PHiD-CV (n=30) or Hepatitis A vaccine (n=30) 60 days apart, were constructed by 16S rRNA gene pyrosequencing of swab specimens collected before vaccination and 180 days after dose 1. Results: Prior to vaccination, Moraxella catarrhalis (median of 12.3%of sequences/subject), Streptococcus pneumoniae (4.4%) and Corynebacterium spp. (5.6%) were the most abundant nasopharyngeal bacterial species. Vaccination with PHiD-CV did not significantly alter the species composition, abundance, or prevalence of known pathogens. Distinct microbiomes were identified based on the abundances of Streptococcus, Moraxella, and Haemophilus species. These microbiomes shifted in composition over the study period and were independent of age, sex, school attendance, antibiotic exposure, and vaccination. Conclusions: Vaccination of children with two doses of PHiD-CV did not significantly alter the nasopharyngeal microbiome. This suggests limited replacement carriage with pathogens other than non-vaccine strains of S. pneumoniae.
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U2 - 10.1371/journal.pone.0128064
DO - 10.1371/journal.pone.0128064
M3 - Article
C2 - 26083474
AN - SCOPUS:84939148132
VL - 10
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 6
M1 - e0128064
ER -