TY - JOUR
T1 - Effects of temperature on glycosyltransferase activity in the plasma membrane of L cells
AU - Setlow, Valerie Petit
AU - Roth, Stephen
AU - Edidin, Michael
N1 - Funding Information:
This work was performed in partial fulfillment of the requirements for the Ph.D. Degree, The Johns Hopkins University. V. P. S. was supported by a training grant, GM-57 to the Department of Biology and by the National Fellowship Fund. The experiments were supported by a grant AM11202 to M. E.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979/6
Y1 - 1979/6
N2 - Derivatives of mouse L cells, cl-ld and LM, are capable of self- or cis-glycosylation. This reaction appears to require interaction of transferase and acceptor by collision in the course of diffusion in the plane of the membrane. The effect of temperature on glycosylation indicates this may well be the case since anomalies in the rate of the reaction, unexpectedly high rates, are found at temperatures below 20 °C. The anomalies are similar to those previously found for lateral diffusion of surface antigens in these cells. Such anomalies are not found for the transfer of galactose by the cells' enzymes to a water-soluble acceptor.
AB - Derivatives of mouse L cells, cl-ld and LM, are capable of self- or cis-glycosylation. This reaction appears to require interaction of transferase and acceptor by collision in the course of diffusion in the plane of the membrane. The effect of temperature on glycosylation indicates this may well be the case since anomalies in the rate of the reaction, unexpectedly high rates, are found at temperatures below 20 °C. The anomalies are similar to those previously found for lateral diffusion of surface antigens in these cells. Such anomalies are not found for the transfer of galactose by the cells' enzymes to a water-soluble acceptor.
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U2 - 10.1016/0014-4827(79)90443-9
DO - 10.1016/0014-4827(79)90443-9
M3 - Article
C2 - 446530
AN - SCOPUS:0018773522
SN - 0014-4827
VL - 121
SP - 55
EP - 61
JO - Experimental cell research
JF - Experimental cell research
IS - 1
ER -