Effects of subperineurial injections of very-long-chain and medium-chain fatty acids into rat sciatic nerve

Ned C. Sacktor, John Griffin, Ann B. Moser, Hugo W. Moser

Research output: Contribution to journalArticlepeer-review

Abstract

[9,10-3H] palmitic (C16:0) and [1-14C] lignoceric (C24:0) acid dissolved in 10 μL of ethanol were injected subperineurially into the sciatic nerve of rats. Both C16:0 and C24:0 were incorporated into lipids, and in most lipid fractions C16:0 incorporation exceeded that of C24:0. Free ceramide and cholesterol ester were the only lipid moieties in which C24:0 incorporation was equal to or greater than that of C16:0. This finding is of particular interest since the very-long-chain fatty acid excess is by far the most striking in the cholesterol ester fraction in adrenoleukodystrophy. Furthermore, incorporation into cerebroside and sulfatide indicates that at least some of the injected fatty acids were metabolized in the Schwann cell. Subperineurial injections of either very-long-chain fatty acids or medium-chain fatty acids into rat sciatic nerve caused demyelination, and this morphological change does not occur following injection of pure solvent.

Original languageEnglish (US)
Pages (from-to)71-83
Number of pages13
JournalNeurochemical Pathology
Volume5
Issue number1
DOIs
StatePublished - Aug 1986

Keywords

  • Adrenoleukodystrophy
  • Schwann cells
  • adrenomyeloneuropathy
  • ceramide
  • cerebroside
  • cholesterol ester
  • demyelination
  • fatty acid, injections of into rat nerves
  • hexacosanoic acid
  • lignoceric acid
  • myelin
  • palmitic acid
  • sciatic nerve
  • subperineurial injection
  • sulfatide

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology

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