Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis (Hymenoptera: Pteromalidae)

Shengzhang Dong, Gong Yin Ye, Peng Cheng Yao, Yao Liang Huang, Xue Xin Chen, Zhi Cheng Shen, Hu Cui

Research output: Contribution to journalArticle

Abstract

A female-specific protein, vitellogenin (Vg), and its corresponding egg vitellin (Vt) are identified in the ectoparasitic wasp Nasonia vitripennis. Both native Vt and Vg have a molecular mass of about 350 kDa, which is composed of two subunits of approximately 190 kDa and 165 kDa under reducing and denaturing conditions (sodium dodecyl sulfatepolyacrylamide gel electrophoresis). An indirect sandwich enzyme-linked immunosorbent assay developed with both monoclonal and polyclonal antibodies against N. vitripennis Vt. Vg was first detected in the hemolymph on the 10th day after parasitism, and was first observed in oocytes on the 12th day. In adults deprived of food, the highest hemolymph Vg level occurred at the time of adult eclosion and the highest level of Vt in ovaries was found at 30 h after eclosion. In contrast, feeding adults with 20% sucrose resulted in the reduction of Vt uptake by ovaries and the extension of life span, but had little effect on Vg production. Deprived of hosts, starvation of female wasps had no significant effects on ovariole growth and oocyte maturation before the wasps died. However, starvation of female wasps supplied with hosts accelerated the wasps laying progeny into hosts, but resulted in a decrease of total progeny production by comparison with wasps fed with 20% sucrose.

Original languageEnglish (US)
Pages (from-to)429-440
Number of pages12
JournalInsect Science
Volume15
Issue number5
DOIs
StatePublished - Oct 1 2008
Externally publishedYes

Fingerprint

Vitellins
Vitellogenesis
vitellin
Nasonia vitripennis
Vitellogenins
Wasps
Hymenoptera
Pteromalidae
ovarian development
vitellogenin
vitellogenesis
Starvation
wasp
starvation
Fertility
fecundity
Hemolymph
hemolymph
Sucrose
eclosion

Keywords

  • Nasonia vitripennis
  • Oocyte
  • Starvation
  • Vitellin
  • Vitellogenin

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Insect Science

Cite this

Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis (Hymenoptera : Pteromalidae). / Dong, Shengzhang; Ye, Gong Yin; Yao, Peng Cheng; Huang, Yao Liang; Chen, Xue Xin; Shen, Zhi Cheng; Cui, Hu.

In: Insect Science, Vol. 15, No. 5, 01.10.2008, p. 429-440.

Research output: Contribution to journalArticle

Dong, Shengzhang ; Ye, Gong Yin ; Yao, Peng Cheng ; Huang, Yao Liang ; Chen, Xue Xin ; Shen, Zhi Cheng ; Cui, Hu. / Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis (Hymenoptera : Pteromalidae). In: Insect Science. 2008 ; Vol. 15, No. 5. pp. 429-440.
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abstract = "A female-specific protein, vitellogenin (Vg), and its corresponding egg vitellin (Vt) are identified in the ectoparasitic wasp Nasonia vitripennis. Both native Vt and Vg have a molecular mass of about 350 kDa, which is composed of two subunits of approximately 190 kDa and 165 kDa under reducing and denaturing conditions (sodium dodecyl sulfatepolyacrylamide gel electrophoresis). An indirect sandwich enzyme-linked immunosorbent assay developed with both monoclonal and polyclonal antibodies against N. vitripennis Vt. Vg was first detected in the hemolymph on the 10th day after parasitism, and was first observed in oocytes on the 12th day. In adults deprived of food, the highest hemolymph Vg level occurred at the time of adult eclosion and the highest level of Vt in ovaries was found at 30 h after eclosion. In contrast, feeding adults with 20{\%} sucrose resulted in the reduction of Vt uptake by ovaries and the extension of life span, but had little effect on Vg production. Deprived of hosts, starvation of female wasps had no significant effects on ovariole growth and oocyte maturation before the wasps died. However, starvation of female wasps supplied with hosts accelerated the wasps laying progeny into hosts, but resulted in a decrease of total progeny production by comparison with wasps fed with 20{\%} sucrose.",
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T1 - Effects of starvation on the vitellogenesis, ovarian development and fecundity in the ectoparasitoid, Nasonia vitripennis (Hymenoptera

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AU - Dong, Shengzhang

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AU - Shen, Zhi Cheng

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