Effects of repeated brief coronary occlusion on regional left ventricular function and dimension in dogs

John M. Nicklas, Lewis C. Becker, Bernadine Healy Bulkley

Research output: Contribution to journalArticlepeer-review


The cumulative effects of repeated, bried episodes of regional ischemia on myocardial function and dimension were examined in 14 open-chest dogs. The left anterior descending coronary artery was occluded for 5 minutes, followed by 10 minutes of reflow, repeated 16 times, and then 1 hour recovery. Systolic function decreased progressively in segments made repetitively ischemic and remained depressed even after 1 hour of recovery. Average systolic shortening decreased 20% from baseline after recovery from the first occlusion, 82% after the 8th, 91% after the 16th, and 104% after the 1 hour recovery (p < 0.015, analysis of variance). End-diastolic segment length progressively increased in regions made repetitively ischemic, lengthening 4% after the first occlusion, 10% after the third occlusion, 19% after the sixteenth occlusion, and 16% after 1 hour of recovery (p < 0.02). Nonischemic end-diastolic segment length also showed a smaller but parallel increase, while nonischemic systolic function showed compensatory improvement. After the dogs were killed, myocardial staining with triphenyl tetrazolium chloride revealed no necrosis. Electron microscopy, performed in 5 dogs, showed scattered mitochondrial swelling in both postischemic and nonischemic regions, but no evidence of irreversible injury. The ratio of myocardial blood flow in the region made repetitively ischemic to nonischemic flow, as measured with microspheres, was 1.00 ± 0.02 before the occlusions and 0.90 ± 0.03 just before death (difference not significant). Thus, in the dog progressively abnormal regional systolic function and regional and global diastolic dilatation can be produced by repetitive, brief, coronary occlusions, which are not associated with histochemical or ultrastructural evidence of myocardial necrosis.

Original languageEnglish (US)
Pages (from-to)473-478
Number of pages6
JournalThe American journal of cardiology
Issue number7
StatePublished - Sep 1 1985

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine


Dive into the research topics of 'Effects of repeated brief coronary occlusion on regional left ventricular function and dimension in dogs'. Together they form a unique fingerprint.

Cite this