Effects of psoralen plus UVA radiation (PUVA) on HIV-1 in human beings: A pilot study

T. D. Horn, W. L. Morison, Homayoon Farzadegan, B. Z. Zmudzka, J. Z. Beer

Research output: Contribution to journalArticle

Abstract

Background: Laboratory data document the activation of the HIV-1 genome on exposure to UV radiation, including PUVA. The overall effects of UV radiation exposure on HIV-1 infection in human beings are unknown. Objective: Our purpose was to observe CD4 cell counts and quantitative markers of HIV-1 load in late-stage HIV-1-infected human beings receiving PUVA for various cutaneous diseases. Methods: Samples of peripheral blood were obtained on days 0, 14, 30, and 60 of PUVA administered in therapeutic doses. Number of CD4+ T lymphocytes was determined by flow cytometry, and HIV-1 load was measured by semiquantitative polymerase chain reaction for viral genome in peripheral blood mononuclear cells, semiquantitative RNA-polymerase chain reaction for HIV-1 RNA in serum, and determination of p24 in serum. Results: No significant changes in the measurements were observed. Conclusion: This study did not detect a deleterious effect on CD4 cell count or HIV-1 load during 2 months of PUVA treatment for patients in late stages of infection, with low CD4 cell counts and high HIV-1 loads.

Original languageEnglish (US)
Pages (from-to)735-740
Number of pages6
JournalJournal of the American Academy of Dermatology
Volume31
Issue number5 I
StatePublished - 1994

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Ficusin
HIV-1
Radiation
CD4 Lymphocyte Count
Polymerase Chain Reaction
Viral Genome
DNA-Directed RNA Polymerases
Serum
Skin Diseases
HIV Infections
Blood Cells
Flow Cytometry
Genome
RNA
T-Lymphocytes

ASJC Scopus subject areas

  • Dermatology

Cite this

Effects of psoralen plus UVA radiation (PUVA) on HIV-1 in human beings : A pilot study. / Horn, T. D.; Morison, W. L.; Farzadegan, Homayoon; Zmudzka, B. Z.; Beer, J. Z.

In: Journal of the American Academy of Dermatology, Vol. 31, No. 5 I, 1994, p. 735-740.

Research output: Contribution to journalArticle

Horn, T. D. ; Morison, W. L. ; Farzadegan, Homayoon ; Zmudzka, B. Z. ; Beer, J. Z. / Effects of psoralen plus UVA radiation (PUVA) on HIV-1 in human beings : A pilot study. In: Journal of the American Academy of Dermatology. 1994 ; Vol. 31, No. 5 I. pp. 735-740.
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