Effects of postnatal hypoxia-ischemia on cholinergic neurons in the developing rat forebrain: choline acetyltransferase immunocytochemistry

Michael V. Johnston, Christopher Hudson

Research output: Contribution to journalArticle

Abstract

We studied the effect of early postnatal hypoxia-ischemia on cholinergic neurons in the developing rat forebrain using immunohistochemistry for choline acetyltransferase (ChAT). In 7-day-old rat pups, hypoxia-ischemia was induced in one cerebral hemisphere by combining unilateral carotid ligation with exposure to 8% oxygen for 2.5 h. This procedure caused brain injury in the hemisphere ipsilateral to ligation, most prominent in the corpus striatum, hippocampus and overlying cortex. In animals sacrificed 2-3 weeks after the insult, at approximately 3 weeks of age, the density of cholinergic cell bodies was slightly higher in the lesioned rostral caudate-putamen than the opposite side (+12%, P < 0.05). In the more caudal portion of caudate-putamen, this effect was greater. In contrast, the size of the cholinergic perikarya in the injured striatum was significantly reduced. Cholinergic neurons in the septum (Ch1, Ch2), globus pallidus and nucleus basalis (Ch4) were relatively unaffected. Considered together with previously reported neurochemical data, these observations suggest that the immature cholinergic neurons are less vulnerable to death from hypoxia-ischemia than other components of the striatum. However, differentiation of surviving cholinergic perikarya and possibly their axonodendritic processes may be disrupted by the early insult.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalDevelopmental Brain Research
Volume34
Issue number1
DOIs
StatePublished - Jul 1987
Externally publishedYes

    Fingerprint

Keywords

  • Caudate-putamen
  • Choline acetyltransferase
  • Cholinergic neuron
  • Hypoxia-ischemia
  • Nucleus basalis
  • Striatum

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Cite this