Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats

Pedro Felipe Silva de Freitas, Leonardo De Castro Duraes, Felipe Augusto Neves Oliveira de Carvalho, Sérgio Andurte Carvalho Duarte, Fabiana Pirani Carneiro, João Batista de Sousa

Research output: Contribution to journalArticle

Abstract

Purpose: To evaluate the effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in a rat model. Methods: Twenty four rats were randomized into three groups (n=8): gasless insufflation ('open', Pressure=0 mmHg), carbon dioxide pneumoperitoneum at 12 mmHg, and helium pneumoperitoneum at 12 mmHg; all lasting 90 minutes. A cystostomy was performed and the bladder was emptied. At the end of the experiment, the urine produced, a blood sample and the left kidney of each animal were collected. The following variables were obtained: serum sodium, potassium, urea and creatinine, urine volume and creatinine. The creatinine clearance was estimated for each animal. The kidneys were stained with hematoxylin and eosin (HE) and evaluated by a pathologist blinded to the groups. Results: The CO2 and Helium groups did not differ in the variables evaluated. Both developed oliguria (p<0.001 vs. gasless). The CO2 group presented hyperkalemia compared to gasless (p=0.05), which did not attain significance in the helium group. Histopathological analysis revealed mild hydropic degeneration and congestion in the three groups, with no significant difference among them. Conclusions: The type of gas resulted in no difference in the variables of renal function and morphology assessed. The increase in serum potassium was only observed with CO2 insufflation suggests a combined effect of elevated intra-abdominal pressure and metabolic effects of pneumoperitoneum.

Original languageEnglish (US)
Pages (from-to)494-498
Number of pages5
JournalActa Cirurgica Brasileira
Volume28
Issue number7
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Helium
Pneumoperitoneum
Carbon Dioxide
Kidney
Creatinine
Insufflation
Potassium
Cystostomy
Urine
Oliguria
Pressure
Hyperkalemia
Hematoxylin
Eosine Yellowish-(YS)
Serum
Urea
Edema
Urinary Bladder
Gases
Sodium

Keywords

  • Artificial
  • Carbon Dioxide
  • Helium
  • Kidney
  • Laparoscopy
  • Pneumoperitoneum
  • Rats
  • Surgery

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

de Freitas, P. F. S., De Castro Duraes, L., de Carvalho, F. A. N. O., Duarte, S. A. C., Carneiro, F. P., & de Sousa, J. B. (2013). Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats. Acta Cirurgica Brasileira, 28(7), 494-498. https://doi.org/10.1590/S0102-86502013000700003

Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats. / de Freitas, Pedro Felipe Silva; De Castro Duraes, Leonardo; de Carvalho, Felipe Augusto Neves Oliveira; Duarte, Sérgio Andurte Carvalho; Carneiro, Fabiana Pirani; de Sousa, João Batista.

In: Acta Cirurgica Brasileira, Vol. 28, No. 7, 01.01.2013, p. 494-498.

Research output: Contribution to journalArticle

de Freitas, Pedro Felipe Silva ; De Castro Duraes, Leonardo ; de Carvalho, Felipe Augusto Neves Oliveira ; Duarte, Sérgio Andurte Carvalho ; Carneiro, Fabiana Pirani ; de Sousa, João Batista. / Effects of pneumoperitoneum with carbon dioxide and helium on renal function and morphology in rats. In: Acta Cirurgica Brasileira. 2013 ; Vol. 28, No. 7. pp. 494-498.
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