Effects of phosphotungstic acid and silicotungstic acid on respiration and integrity of rat liver mitochondria

William A. Catterall, Peter L. Pedersen

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Phosphotungstic acid and silicotungstic acid, when employed at low concentrations (≤ 0.2 mg/mg protein), were found to markedly inhibit mitochondrial respiration stimulated by either ADP or 2,4-dinitrophenol. At higher concentrations these negative staining agents were found to solubilize significant amounts of mitochondrial protein. The results clearly show that PTA and STA are not biochemically inert and probably have more than a single mitochondrial site of action.

Original languageEnglish (US)
Pages (from-to)400-405
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume38
Issue number3
DOIs
StatePublished - Feb 6 1970

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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