Phosphotungstic acid and silicotungstic acid, when employed at low concentrations (≤ 0.2 mg/mg protein), were found to markedly inhibit mitochondrial respiration stimulated by either ADP or 2,4-dinitrophenol. At higher concentrations these negative staining agents were found to solubilize significant amounts of mitochondrial protein. The results clearly show that PTA and STA are not biochemically inert and probably have more than a single mitochondrial site of action.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 6 1970|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology