Effects of pH Variability on Peracetic Acid Reduction of Human Norovirus GI, GII RNA, and Infectivity Plus RNA Reduction of Selected Surrogates

Nathan Dunkin, Caroline Coulter, Shih Chi Weng, Joseph G. Jacangelo, Kellogg J. Schwab

Research output: Contribution to journalArticlepeer-review

Abstract

With increasing interest in peracetic acid (PAA) as a disinfectant in water treatment processes, this study determined PAA treatment effects on human noroviruses (hNoVs) genotype I (GI) and genotype II (GII) as well as effects on bacteriophage MS2 and murine norovirus (MNV) in relation to pH. Across all pH conditions, PAA achieved between 0.2 and 2.5 log 10 reduction of hNoVs over 120 min contact time in buffer solution as measured by reverse transcription-qPCR (RT-qPCR). The PAA treatments produced similar RT-qPCR reductions of MS2 and MNV, in the range of 0.2–2.7 log 10 . Infectivity assays achieved > 4 log 10 reduction of both MS2 and MNV in buffer solution after 120 min contact time. Comparing PAA activity across varying pH, disinfection at pH 8.5, in general, resulted in less reduction of infectivity and molecular signals compared to pH conditions of 6.5 and 7.5. This difference was most pronounced for reductions in infectivity of MNV and MS2, with as much as 2.7 log 10 less reduction at pH 8.5 relative to lower pH conditions. This study revealed that PAA was an effective disinfectant for treatment of hNoV GI and GII, MS2 and MNV, with greatest virus reduction observed for MS2 and MNV infectivity. RT-qPCR reductions of MS2 and MNV were lower than concurrent MS2 and MNV infectivity reductions, suggesting that observed hNoV RT-qPCR reductions may underestimate reductions in hNoV infectivity achieved by PAA. Although virus disinfection by PAA occurred at all evaluated pH levels, PAA is most effective at pH 6.5–7.5.

Original languageEnglish (US)
Pages (from-to)76-89
Number of pages14
JournalFood and Environmental Virology
Volume11
Issue number1
DOIs
StatePublished - Mar 15 2019

Keywords

  • Disinfection
  • Human norovirus
  • MS2 bacteriophage
  • Mouse norovirus
  • Peracetic acid
  • pH

ASJC Scopus subject areas

  • Epidemiology
  • Food Science
  • Health, Toxicology and Mutagenesis
  • Virology

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