Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites

Lee J Martin, Joe D. Morse, Gary A. Pritchard, Young H. Kim, Adam Anthony

Research output: Contribution to journalArticle

Abstract

Neuronal nucleic acid responses were examined within the rat thalamic ventro-basal nuclear complex (VBC) and nucleus reticularis (NR) following single intraperitoneal injections of the central muscarinic-cholinergic (M2) receptor agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric azure B and Feulgen staining of brain sections, scanning-integrating cytophotometry was used to quantify azure B-ribonucleic acid (RNA) content, Feulgen-DNA levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) of neurons on an individual basis. Changes in neuronal nucleolar volume were also determined histometrically. Within the VBC, oxotremorine produced marked dose-dependent elevations in neuronal RNA content and nucleolar volume with increased F-DNA yield (chromatin activation) in a proportion of VBC neurons. In contrast, within the NR, oxotremorine elicited reductions in RNA levels, F-DNA yield and nucleolar volume. The data demonstrate that oxotremorine-induced central muscarinic receptor stimulation is associated with metabolic correlates of thalamic VBC neuroexcitation and NR neuron depression. The overall study lends further credence to the hypothesis that muscarinic-cholinergic mechanisms are operative within the mammalian thalamus.

Original languageEnglish (US)
Pages (from-to)159-169
Number of pages11
JournalJournal of the Neurological Sciences
Volume86
Issue number2-3
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Oxotremorine
Cholinergic Receptors
Chromatin
RNA
DNA
Neurons
Cholinergic Agents
Muscarinic M2 Receptors
Cytophotometry
Muscarinic Receptors
Intraperitoneal Injections
Thalamus
Nucleic Acids
Hydrolysis
Staining and Labeling
Acids
Brain
Azure B

Keywords

  • Central muscarinic stimulation
  • Chromatin
  • Muscarinic receptor agonists
  • Oxotremorine
  • Quantitative Feulgen-DNA and azure B-RNA cytophotometry
  • RNA
  • Thalamic cholinoceptive sites

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

Cite this

Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites. / Martin, Lee J; Morse, Joe D.; Pritchard, Gary A.; Kim, Young H.; Anthony, Adam.

In: Journal of the Neurological Sciences, Vol. 86, No. 2-3, 1988, p. 159-169.

Research output: Contribution to journalArticle

Martin, Lee J ; Morse, Joe D. ; Pritchard, Gary A. ; Kim, Young H. ; Anthony, Adam. / Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites. In: Journal of the Neurological Sciences. 1988 ; Vol. 86, No. 2-3. pp. 159-169.
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