Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites

Lee J. Martin; Joe D. Morse; Gary A. Pritchard; Young H. Kim; Adam Anthony

doi:10.1016/0022-510X(88)90095-0

Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites

Lee J. Martin, Joe D. Morse, Gary A. Pritchard, Young H. Kim, Adam Anthony

Research output: Contribution to journal › Article › peer-review

Abstract

Neuronal nucleic acid responses were examined within the rat thalamic ventro-basal nuclear complex (VBC) and nucleus reticularis (NR) following single intraperitoneal injections of the central muscarinic-cholinergic (M2) receptor agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric azure B and Feulgen staining of brain sections, scanning-integrating cytophotometry was used to quantify azure B-ribonucleic acid (RNA) content, Feulgen-DNA levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) of neurons on an individual basis. Changes in neuronal nucleolar volume were also determined histometrically. Within the VBC, oxotremorine produced marked dose-dependent elevations in neuronal RNA content and nucleolar volume with increased F-DNA yield (chromatin activation) in a proportion of VBC neurons. In contrast, within the NR, oxotremorine elicited reductions in RNA levels, F-DNA yield and nucleolar volume. The data demonstrate that oxotremorine-induced central muscarinic receptor stimulation is associated with metabolic correlates of thalamic VBC neuroexcitation and NR neuron depression. The overall study lends further credence to the hypothesis that muscarinic-cholinergic mechanisms are operative within the mammalian thalamus.

Original language	English (US)
Pages (from-to)	159-169
Number of pages	11
Journal	Journal of the Neurological Sciences
Volume	86
Issue number	2-3
DOIs	https://doi.org/10.1016/0022-510X(88)90095-0
State	Published - Sep 1988
Externally published	Yes

Keywords

Central muscarinic stimulation
Chromatin
Muscarinic receptor agonists
Oxotremorine
Quantitative Feulgen-DNA and azure B-RNA cytophotometry
RNA
Thalamic cholinoceptive sites

ASJC Scopus subject areas

Neurology
Clinical Neurology

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10.1016/0022-510X(88)90095-0

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title = "Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites",

abstract = "Neuronal nucleic acid responses were examined within the rat thalamic ventro-basal nuclear complex (VBC) and nucleus reticularis (NR) following single intraperitoneal injections of the central muscarinic-cholinergic (M2) receptor agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric azure B and Feulgen staining of brain sections, scanning-integrating cytophotometry was used to quantify azure B-ribonucleic acid (RNA) content, Feulgen-DNA levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) of neurons on an individual basis. Changes in neuronal nucleolar volume were also determined histometrically. Within the VBC, oxotremorine produced marked dose-dependent elevations in neuronal RNA content and nucleolar volume with increased F-DNA yield (chromatin activation) in a proportion of VBC neurons. In contrast, within the NR, oxotremorine elicited reductions in RNA levels, F-DNA yield and nucleolar volume. The data demonstrate that oxotremorine-induced central muscarinic receptor stimulation is associated with metabolic correlates of thalamic VBC neuroexcitation and NR neuron depression. The overall study lends further credence to the hypothesis that muscarinic-cholinergic mechanisms are operative within the mammalian thalamus.",

keywords = "Central muscarinic stimulation, Chromatin, Muscarinic receptor agonists, Oxotremorine, Quantitative Feulgen-DNA and azure B-RNA cytophotometry, RNA, Thalamic cholinoceptive sites",

author = "Martin, {Lee J.} and Morse, {Joe D.} and Pritchard, {Gary A.} and Kim, {Young H.} and Adam Anthony",

year = "1988",

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doi = "10.1016/0022-510X(88)90095-0",

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T1 - Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites

AU - Martin, Lee J.

AU - Morse, Joe D.

AU - Pritchard, Gary A.

AU - Kim, Young H.

AU - Anthony, Adam

PY - 1988/9

Y1 - 1988/9

N2 - Neuronal nucleic acid responses were examined within the rat thalamic ventro-basal nuclear complex (VBC) and nucleus reticularis (NR) following single intraperitoneal injections of the central muscarinic-cholinergic (M2) receptor agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric azure B and Feulgen staining of brain sections, scanning-integrating cytophotometry was used to quantify azure B-ribonucleic acid (RNA) content, Feulgen-DNA levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) of neurons on an individual basis. Changes in neuronal nucleolar volume were also determined histometrically. Within the VBC, oxotremorine produced marked dose-dependent elevations in neuronal RNA content and nucleolar volume with increased F-DNA yield (chromatin activation) in a proportion of VBC neurons. In contrast, within the NR, oxotremorine elicited reductions in RNA levels, F-DNA yield and nucleolar volume. The data demonstrate that oxotremorine-induced central muscarinic receptor stimulation is associated with metabolic correlates of thalamic VBC neuroexcitation and NR neuron depression. The overall study lends further credence to the hypothesis that muscarinic-cholinergic mechanisms are operative within the mammalian thalamus.

AB - Neuronal nucleic acid responses were examined within the rat thalamic ventro-basal nuclear complex (VBC) and nucleus reticularis (NR) following single intraperitoneal injections of the central muscarinic-cholinergic (M2) receptor agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric azure B and Feulgen staining of brain sections, scanning-integrating cytophotometry was used to quantify azure B-ribonucleic acid (RNA) content, Feulgen-DNA levels, and changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) of neurons on an individual basis. Changes in neuronal nucleolar volume were also determined histometrically. Within the VBC, oxotremorine produced marked dose-dependent elevations in neuronal RNA content and nucleolar volume with increased F-DNA yield (chromatin activation) in a proportion of VBC neurons. In contrast, within the NR, oxotremorine elicited reductions in RNA levels, F-DNA yield and nucleolar volume. The data demonstrate that oxotremorine-induced central muscarinic receptor stimulation is associated with metabolic correlates of thalamic VBC neuroexcitation and NR neuron depression. The overall study lends further credence to the hypothesis that muscarinic-cholinergic mechanisms are operative within the mammalian thalamus.

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KW - Chromatin

KW - Muscarinic receptor agonists

KW - Oxotremorine

KW - Quantitative Feulgen-DNA and azure B-RNA cytophotometry

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Effects of oxotremorine on neuronal RNA and chromatin in thalamic cholinoceptive sites

Abstract

Keywords

ASJC Scopus subject areas

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