Effects of nitric oxide on human and canine prostates

Masayuki Takeda, Rui Tang, Ellen Shapiro, Arthur Burnett, Herbert Lepor

Research output: Contribution to journalArticle

Abstract

Objectives: To determine whether nitric oxide (NO) is a mediator of prostatic smooth muscle activity. Methods: Pharmacologic experiments using electrical field stimulation (EFS) were performed on strips of human and canine prostate. Results: EFS alone elicited frequency-dependent contractions in preparations of human and canine prostates. The greatest contractile activity was achieved at 30 Hz. In the presence of 10-5 M guanethidine (GUA) and 2 × 10-1 M atropine (ATR), EFS elicited relaxation of canine prostate strips relative to baseline tension. A weak biphasic response consisting of initial relaxation and subsequent contraction relative to baseline tension was observed in the human prostate strips exposed to similar conditions. The smooth muscle activity observed in the presence of GUA plus ATR was attributed to nonadrenergic, noncholinergic (NANC) nerve transmission. 10-4 M L-NG-nitroarginine methylester (NAME) significantly increased EFS-elicited NANC smooth muscle activity both in human and canine prostates. L-arginine, 10-2 M, reversed the effect of L-NAME in human and canine prostates. Sodium nitroprusside, 10-4 M, a donor of NO, caused relaxation of both human and canine prostates. The mean magnitude of the relaxant response/cross-sectional area in human prostate (2.64 ± 0.4 g/cm2) was significantly greater than in the canine prostate (1.09 ± 0.17 g/cm2) (P <0.005). Conclusions: These results provide compelling evidence that NO plays a role in mediating contractile function of human and canine prostates.

Original languageEnglish (US)
Pages (from-to)440-446
Number of pages7
JournalUrology
Volume45
Issue number3
DOIs
StatePublished - 1995

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Canidae
Prostate
Nitric Oxide
Electric Stimulation
Smooth Muscle
Guanethidine
Nitroarginine
Atropine
Nitric Oxide Donors
Nitroprusside
Arginine

ASJC Scopus subject areas

  • Urology

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Effects of nitric oxide on human and canine prostates. / Takeda, Masayuki; Tang, Rui; Shapiro, Ellen; Burnett, Arthur; Lepor, Herbert.

In: Urology, Vol. 45, No. 3, 1995, p. 440-446.

Research output: Contribution to journalArticle

Takeda, M, Tang, R, Shapiro, E, Burnett, A & Lepor, H 1995, 'Effects of nitric oxide on human and canine prostates', Urology, vol. 45, no. 3, pp. 440-446. https://doi.org/10.1016/S0090-4295(99)80013-2
Takeda, Masayuki ; Tang, Rui ; Shapiro, Ellen ; Burnett, Arthur ; Lepor, Herbert. / Effects of nitric oxide on human and canine prostates. In: Urology. 1995 ; Vol. 45, No. 3. pp. 440-446.
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