Effects of multiple freeze thaws and various temperatures on the reactivity of human immunodeficiency virus antibody using three detection assays

Donald R. Fipps, James J. Damato, Brenda Brandt, Donald S. Burke

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of multiple freeze-thaw cycles and various temperatures (-20°C, 4°C, 25°C, 37°C) on the reactivity of human immunodeficiency virus (HIV) antibodies were evaluated using current ELISA, recombinant, and Western blot methodologies. Twenty consecutive freeze-thaw cycles and storage of specimens at -20°C and 4°C for 57 days resulted in no loss of HIV antibody reactivity nor false positive samples. Maintenance of clinical specimens at 25°C and 37°C for 57 days resulted in some loss of HIV antibody reactivity, but all positive and weakly reacting samples remained positive, and negative samples were unaffected.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalJournal of Virological Methods
Volume20
Issue number2
DOIs
StatePublished - Jun 1988

Keywords

  • ELISA
  • Freeze-thaw cycles
  • HIV antibody reactivity
  • Recombinant
  • Specimens, positive and negative
  • Western blot

ASJC Scopus subject areas

  • Virology

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