Effects of monoclonal antibodies to the α and β chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions

D. W. Dongworth, F. M. Gotch, J. E K Hildreth, A. Morris, A. J. McMichael

    Research output: Contribution to journalArticle

    Abstract

    The ability of two antibodies, one specific for the α chain, p180, and the other for the β chain, p95, of the human lymphocyte function-associated (LFA-1) antigens, to inhibit T cell function was measured. Both antibodies inhibited T cell-mediated lysis of virus-infected target cells and of K562 cells. Only the anti-β chain antibody inhibited natural killer cell lysis of K562. The antibodies inhibited cytotoxic T lymphocyte cell (CTL) lysis of HLA-mismatched target cells in the presence of concanavalin A at 6.25-12.5 μg/ml, but at higher doses of Con A no inhibition was seen. When the lytic process was divided into calcium-independent (adherence) and -dependent (lysis) steps the antibodies were found to block at the initial step of conjugate formation. The effects of these antibodies on T cell proliferative responses showed that responses to antigens, alloantigens, mitogens and anti-CD3 (UCHT1) antibody were greatly inhibited. All of these responses are adherent cell dependent and proliferation of adherent cell-depleted mononuclear cells to Sepharose-coupled UCHT1 was not inhibited by anti-LFA-1 antibodies. Proliferation to paired anti-CD2 (T11) antibodies was also only weakly inhibited. Release of interferon-γ by CTL on contact with target cells was also inhibited by anti-LFA antibody. These results are evidence that the LFA antigen is necessary for a nonspecific interaction with antigen-presenting cells that is essential for activation of T cells through the CD3-T cell receptor complex.

    Original languageEnglish (US)
    Pages (from-to)888-892
    Number of pages5
    JournalEuropean Journal of Immunology
    Volume15
    Issue number9
    DOIs
    StatePublished - 1985

    Fingerprint

    Lymphocyte Function-Associated Antigen-1
    Monoclonal Antibodies
    Lymphocytes
    T-Lymphocytes
    Antibodies
    Cytotoxic T-Lymphocytes
    Antigens
    Anti-Idiotypic Antibodies
    Isoantigens
    K562 Cells
    H-type-1 antigen
    Antigen-Presenting Cells
    Concanavalin A
    T-Cell Antigen Receptor
    Mitogens
    Natural Killer Cells
    Sepharose
    Interferons
    Cell Proliferation
    Viruses

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Effects of monoclonal antibodies to the α and β chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions. / Dongworth, D. W.; Gotch, F. M.; Hildreth, J. E K; Morris, A.; McMichael, A. J.

    In: European Journal of Immunology, Vol. 15, No. 9, 1985, p. 888-892.

    Research output: Contribution to journalArticle

    Dongworth, D. W. ; Gotch, F. M. ; Hildreth, J. E K ; Morris, A. ; McMichael, A. J. / Effects of monoclonal antibodies to the α and β chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions. In: European Journal of Immunology. 1985 ; Vol. 15, No. 9. pp. 888-892.
    @article{5601836c602745ed8c06cf0337d09f78,
    title = "Effects of monoclonal antibodies to the α and β chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions",
    abstract = "The ability of two antibodies, one specific for the α chain, p180, and the other for the β chain, p95, of the human lymphocyte function-associated (LFA-1) antigens, to inhibit T cell function was measured. Both antibodies inhibited T cell-mediated lysis of virus-infected target cells and of K562 cells. Only the anti-β chain antibody inhibited natural killer cell lysis of K562. The antibodies inhibited cytotoxic T lymphocyte cell (CTL) lysis of HLA-mismatched target cells in the presence of concanavalin A at 6.25-12.5 μg/ml, but at higher doses of Con A no inhibition was seen. When the lytic process was divided into calcium-independent (adherence) and -dependent (lysis) steps the antibodies were found to block at the initial step of conjugate formation. The effects of these antibodies on T cell proliferative responses showed that responses to antigens, alloantigens, mitogens and anti-CD3 (UCHT1) antibody were greatly inhibited. All of these responses are adherent cell dependent and proliferation of adherent cell-depleted mononuclear cells to Sepharose-coupled UCHT1 was not inhibited by anti-LFA-1 antibodies. Proliferation to paired anti-CD2 (T11) antibodies was also only weakly inhibited. Release of interferon-γ by CTL on contact with target cells was also inhibited by anti-LFA antibody. These results are evidence that the LFA antigen is necessary for a nonspecific interaction with antigen-presenting cells that is essential for activation of T cells through the CD3-T cell receptor complex.",
    author = "Dongworth, {D. W.} and Gotch, {F. M.} and Hildreth, {J. E K} and A. Morris and McMichael, {A. J.}",
    year = "1985",
    doi = "10.1002/eji.1830150905",
    language = "English (US)",
    volume = "15",
    pages = "888--892",
    journal = "European Journal of Immunology",
    issn = "0014-2980",
    publisher = "Wiley-VCH Verlag",
    number = "9",

    }

    TY - JOUR

    T1 - Effects of monoclonal antibodies to the α and β chains of the human lymphocyte function-associated (H-LFA-1) antigen on T lymphocyte functions

    AU - Dongworth, D. W.

    AU - Gotch, F. M.

    AU - Hildreth, J. E K

    AU - Morris, A.

    AU - McMichael, A. J.

    PY - 1985

    Y1 - 1985

    N2 - The ability of two antibodies, one specific for the α chain, p180, and the other for the β chain, p95, of the human lymphocyte function-associated (LFA-1) antigens, to inhibit T cell function was measured. Both antibodies inhibited T cell-mediated lysis of virus-infected target cells and of K562 cells. Only the anti-β chain antibody inhibited natural killer cell lysis of K562. The antibodies inhibited cytotoxic T lymphocyte cell (CTL) lysis of HLA-mismatched target cells in the presence of concanavalin A at 6.25-12.5 μg/ml, but at higher doses of Con A no inhibition was seen. When the lytic process was divided into calcium-independent (adherence) and -dependent (lysis) steps the antibodies were found to block at the initial step of conjugate formation. The effects of these antibodies on T cell proliferative responses showed that responses to antigens, alloantigens, mitogens and anti-CD3 (UCHT1) antibody were greatly inhibited. All of these responses are adherent cell dependent and proliferation of adherent cell-depleted mononuclear cells to Sepharose-coupled UCHT1 was not inhibited by anti-LFA-1 antibodies. Proliferation to paired anti-CD2 (T11) antibodies was also only weakly inhibited. Release of interferon-γ by CTL on contact with target cells was also inhibited by anti-LFA antibody. These results are evidence that the LFA antigen is necessary for a nonspecific interaction with antigen-presenting cells that is essential for activation of T cells through the CD3-T cell receptor complex.

    AB - The ability of two antibodies, one specific for the α chain, p180, and the other for the β chain, p95, of the human lymphocyte function-associated (LFA-1) antigens, to inhibit T cell function was measured. Both antibodies inhibited T cell-mediated lysis of virus-infected target cells and of K562 cells. Only the anti-β chain antibody inhibited natural killer cell lysis of K562. The antibodies inhibited cytotoxic T lymphocyte cell (CTL) lysis of HLA-mismatched target cells in the presence of concanavalin A at 6.25-12.5 μg/ml, but at higher doses of Con A no inhibition was seen. When the lytic process was divided into calcium-independent (adherence) and -dependent (lysis) steps the antibodies were found to block at the initial step of conjugate formation. The effects of these antibodies on T cell proliferative responses showed that responses to antigens, alloantigens, mitogens and anti-CD3 (UCHT1) antibody were greatly inhibited. All of these responses are adherent cell dependent and proliferation of adherent cell-depleted mononuclear cells to Sepharose-coupled UCHT1 was not inhibited by anti-LFA-1 antibodies. Proliferation to paired anti-CD2 (T11) antibodies was also only weakly inhibited. Release of interferon-γ by CTL on contact with target cells was also inhibited by anti-LFA antibody. These results are evidence that the LFA antigen is necessary for a nonspecific interaction with antigen-presenting cells that is essential for activation of T cells through the CD3-T cell receptor complex.

    UR - http://www.scopus.com/inward/record.url?scp=0021926120&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0021926120&partnerID=8YFLogxK

    U2 - 10.1002/eji.1830150905

    DO - 10.1002/eji.1830150905

    M3 - Article

    C2 - 3930261

    AN - SCOPUS:0021926120

    VL - 15

    SP - 888

    EP - 892

    JO - European Journal of Immunology

    JF - European Journal of Immunology

    SN - 0014-2980

    IS - 9

    ER -