Effects of microtubule stabilization and destabilization on tau immunoreactivity in cultured hippocampal neurons

Mark P. Mattson

Research output: Contribution to journalArticle

Abstract

Tau immunoreactivity is altered in neurofibrillary tangles (NFT) and degenerating neurites in Alzheimer's disease (AD). In addition, cytoskeletal proteins including tau are excessively phosphorylated in AD. Previous data indicated that calcium influx can cause antigenic changes in tau in cultured rat hippocampal and human cortical neurons similar to those seen in NFT. The present study used cultured hippocampal neurons to test the hypothesis that disruption of microtubules is a key event leading to altered antigenic properties of tau that result from calcium influx. As previously reported, we found that glutamate (100-500 μM) and calcium ionophore A23187 (0.5-1 μM) elevated intraneuronal calcium levels and caused a reduction in microtubules, a marked increase in staining with Alz-50 and 5E2, and a decrease in tau-1 immunoreactivity. The microtubule-disrupting agent colchicine (1 μM) caused increased immunoreactivity of neurons towards tau antibodies Alz-50 and 5E2, and these effects of colchicine occurred in the absence of an increase in intracellular calcium levels. The microtubule-stabilizing drug taxol (100 nM) reduced neuronal immunoreactivity towards Alz-50 and 5E2 in untreated cultures and in cultures exposed to glutamate or A23187. Western blot analysis indicated that A23187 caused a reduction in tau levels which was partially prevented by taxol, suggesting that tau associated with microtubules is less susceptible to calcium-mediated degradation. Acid phosphatase treatment increased neuronal immunoreactivity towards tau-1 and reduced immunoreactivity towards Alz-50. The calcium-induced alterations in tau immunoreactivity were, and the colchicine-induced alterations were not, affected by acid phosphatase treatment. Taken together, the data indicate that microtubule depolymerization can cause antigenic changes in tau similar to those seen in NFT independently of an increase in intraneuronal calcium levels. Stabilization of microtubules prevented the antigenic changes in tau suggesting that microtubules affect the availability and/or properties of epitopes on tau that are recognized by antibodies that stain NFT.

Original languageEnglish (US)
Pages (from-to)107-118
Number of pages12
JournalBrain Research
Volume582
Issue number1
DOIs
StatePublished - Jun 5 1992
Externally publishedYes

Fingerprint

Microtubules
Neurons
Neurofibrillary Tangles
Calcium
Calcimycin
Colchicine
Acid Phosphatase
Paclitaxel
Glutamic Acid
Alzheimer Disease
Cytoskeletal Proteins
Calcium Ionophores
Antibodies
Neurites
Epitopes
Coloring Agents
Western Blotting
Staining and Labeling
Pharmaceutical Preparations

Keywords

  • Alzheimer's disease
  • Calcium
  • Colchicine
  • Excitatory amino acid
  • Neurofibrillary tangle
  • Phosphorylation
  • Taxol

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Effects of microtubule stabilization and destabilization on tau immunoreactivity in cultured hippocampal neurons. / Mattson, Mark P.

In: Brain Research, Vol. 582, No. 1, 05.06.1992, p. 107-118.

Research output: Contribution to journalArticle

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