Effects of methylflavonolamine on free intracellular calcium in isolated embryonic rat brain cells

Wen Zhen Duan, Yun Zhao Tang

Research output: Contribution to journalArticlepeer-review

Abstract

AIM: To observe the effects of methylflavonolamine (MFA) on free intracellular calcium concentration ([Ca2+](i)) of isolated embryonic rat brain cells in presence and absence of high extracellular potassium and L- glutamate. METHODS: [Ca2+](i) was measured in a spectrofluorophotometer by preloading the cells with calcium sensitive fluorescent indicator Fura 2-AM. RESULTS: Resting [Ca2+](i) was 197 ± 20 nmol · L-1 (n = 44) in the presence of Ca2+ 1.3 mmol · L-1 in Hanks' solution. MFA 0.15 mmol · L- 1 had no effect on the resting [Ca2+](i). When extracellular Ca2+ was 1.3 mmol · L-1, MFA (0.03 - 0.3 mmol · L-1) concentration-dependently inhibited the [Ca2+](i) elevation induced by high extracellular potassium, with an IC50 value of 0.14 (95 % confidence limits: 0.05-0.42) mmol · L- 1. At higher concentration (0.15 - 0.30 mmol · L-1), MFA decreased L- glutamate-induced [Ca2+](i) elevation, with an IC50 of 0.20 (95 % confidence limits: 0.01-3.40) mmol · L-1. CONCLUSION: MFA inhibited Ca2+ influx through voltage-dependent calcium channel and, at higher concentration, through receptor-operated calcium channel in the embryonic rat brain cells.

Original languageEnglish (US)
Pages (from-to)305-308
Number of pages4
JournalActa Pharmacologica Sinica
Volume17
Issue number4
StatePublished - Jul 1 1996
Externally publishedYes

Keywords

  • Fura-2
  • brain
  • calcium
  • cultured calls
  • flunarizine
  • glutamate
  • methylflavonolamine

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'Effects of methylflavonolamine on free intracellular calcium in isolated embryonic rat brain cells'. Together they form a unique fingerprint.

Cite this