Effects of liposome encapsulated hemoglobin on the reticuloendothelial system

Mary Catherine Beach, J. Morley, L. Spiryda, S. B. Weinstock

Research output: Contribution to journalArticle

Abstract

The effects of liposome encapsulated hemoglobin (LEH) wwew measured on i) colloidal carbon clearance in the rat in vivo and in the isolated perfused liver, ii) histological changes in the liver, and iii) Kupffer cell (KC) function using magnetomery. i) Rats (240-350 gm) were injected i.v. with LEH, empty liposomes (LIP), or Krebs Ringer bicarbonate (KRB) (10% or 25% of blood volume). Carbon clearance was measured 2, 12, 24 hr or 2 wk post-treatment. For in vivo studies, rats were injected with carbon (0.2 ml/100 gms b.w.) and 0.075 ml blood samples were taken from the jugular vein at 1, 3, 5, 7, 10, 20, 30, 45, 60 min. Perfused liver studies were conducted 2 hr or 2 wk post-LEH treatment. Carbon (0.2 ml/100 gm b.w.) was added to the perfusate and 1 ml samples taken at 1, 3, 5, 7, 10, 20, 30, 45, 60 min. Carbon was measured at A695. Phagocytic index, k, was calculated according to the equation A = Aoe-kt. The area under the clearance curve from 0 to 50 minutes (AUC50) was measured and reflects the capacity of the liver or RES to clear carbon. ii). At the end of each in vivo experiment, livers were rinsed with KRB via the portal vein and fixed with 2% glutaraldehyde. iii). In separate previously described (J. Appl. Physiol. 65:4; 1811-1820, 1988). In vivo k-values were 2-3 times lower in LEH and LIP groups than in KRB controls at 2 and 12 hr post-treatment. By 24 hr, all k's were similar. K's were the same in all groups in the perfused liver experiments. In vivo and perfused liver AUC50's were the same in LEH group as in controls. Magnetometric studies showed a decrease in phagosomal motion in KC's in the LEH-treated rats at 2 and 24 hours that returned to control levels at 2 wks. Histological examination showed some KC and hepatocyte vacuolization and mild infiltration of lymphocytes in LEH- and LIP-treated rats. Thus, LEH has some deleterious effects on KC function, but overall RES clearance capacity appears unaffected.

Original languageEnglish (US)
Pages (from-to)352
Number of pages1
JournalBiomaterials, Artificial Cells, and Immobilization Biotechnology
Volume19
Issue number2
StatePublished - 1991
Externally publishedYes

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Mononuclear Phagocyte System
Liposomes
Hemoglobin
Hemoglobins
Liver
Carbon
Rats
Kupffer Cells
Bicarbonates
Blood
Lymphocytes
Level control
Jugular Veins
Glutaral
Portal Vein
Blood Volume
Infiltration
Area Under Curve
Hepatocytes
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of liposome encapsulated hemoglobin on the reticuloendothelial system. / Beach, Mary Catherine; Morley, J.; Spiryda, L.; Weinstock, S. B.

In: Biomaterials, Artificial Cells, and Immobilization Biotechnology, Vol. 19, No. 2, 1991, p. 352.

Research output: Contribution to journalArticle

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abstract = "The effects of liposome encapsulated hemoglobin (LEH) wwew measured on i) colloidal carbon clearance in the rat in vivo and in the isolated perfused liver, ii) histological changes in the liver, and iii) Kupffer cell (KC) function using magnetomery. i) Rats (240-350 gm) were injected i.v. with LEH, empty liposomes (LIP), or Krebs Ringer bicarbonate (KRB) (10{\%} or 25{\%} of blood volume). Carbon clearance was measured 2, 12, 24 hr or 2 wk post-treatment. For in vivo studies, rats were injected with carbon (0.2 ml/100 gms b.w.) and 0.075 ml blood samples were taken from the jugular vein at 1, 3, 5, 7, 10, 20, 30, 45, 60 min. Perfused liver studies were conducted 2 hr or 2 wk post-LEH treatment. Carbon (0.2 ml/100 gm b.w.) was added to the perfusate and 1 ml samples taken at 1, 3, 5, 7, 10, 20, 30, 45, 60 min. Carbon was measured at A695. Phagocytic index, k, was calculated according to the equation A = Aoe-kt. The area under the clearance curve from 0 to 50 minutes (AUC50) was measured and reflects the capacity of the liver or RES to clear carbon. ii). At the end of each in vivo experiment, livers were rinsed with KRB via the portal vein and fixed with 2{\%} glutaraldehyde. iii). In separate previously described (J. Appl. Physiol. 65:4; 1811-1820, 1988). In vivo k-values were 2-3 times lower in LEH and LIP groups than in KRB controls at 2 and 12 hr post-treatment. By 24 hr, all k's were similar. K's were the same in all groups in the perfused liver experiments. In vivo and perfused liver AUC50's were the same in LEH group as in controls. Magnetometric studies showed a decrease in phagosomal motion in KC's in the LEH-treated rats at 2 and 24 hours that returned to control levels at 2 wks. Histological examination showed some KC and hepatocyte vacuolization and mild infiltration of lymphocytes in LEH- and LIP-treated rats. Thus, LEH has some deleterious effects on KC function, but overall RES clearance capacity appears unaffected.",
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