Effects of Kv1.1 channel glycosylation on C-type inactivation and simulated action potentials

Jhon J. Sutachan, Itaru Watanabe, Jing Zhu, Allan Gottschalk, Esperanza Recio-Pinto, William B. Thornhill

Research output: Contribution to journalArticle

Abstract

Kv1.1 channels are brain glycoproteins that play an important role in repolarization of action potentials. In previous work, we showed that lack of N-glycosylation, particularly lack of sialylation, of Kv1.1 affected its macroscopic gating properties and slowed activation and C-type inactivation kinetics and produced a depolarized shift in the steady-state activation curve. In our current study, we used single channel analysis to investigate voltage-independent C-type inactivation in both Kv1.1 and Kv1.1N207Q, a glycosylation mutant. Both channels underwent brief and long-lived closures, and the lifetime and frequency of the long-lived closed states were voltage-independent and similar for both channels. We found that, as in macroscopic measurements, Kv1.1N207Q exhibited a ∼8 mV positive shift in its single channel fractional open time (fo) and a shallower fo-voltage slope compared with Kv1.1. Data suggested that C-type inactivation reflected the equilibration time with at least two slow voltage-independent long-lived closed states that followed the rapid activation process. In addition, data simulation indicated that the C-type inactivation process reflected the equilibration time between the open state and at least two long-lived closed states. Moreover, the faster macroscopic current decay in Kv1.1 mostly reflected a slower equilibration time in these channels as compared with Kv1.1N207Q. Finally, action potential simulations indicated that the N207Q mutation broaden the action potential and decreased the interspike interval. The shape of the action potential was not significantly affected by C-type inactivation, however, for a given channel, C-type inactivation increased the interspike interval. Data and simulations suggested that excitable cells could use differences in K + channel glycosylation degree as an additional mechanism to increase channel functional diversity which could modify cell excitability.

Original languageEnglish (US)
Pages (from-to)30-43
Number of pages14
JournalBrain Research
Volume1058
Issue number1-2
DOIs
StatePublished - Oct 5 2005

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Glycosylation
Action Potentials
Glycoproteins
Mutation
Brain

Keywords

  • Action potential simulation
  • Glycosylation
  • Single K channel

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effects of Kv1.1 channel glycosylation on C-type inactivation and simulated action potentials. / Sutachan, Jhon J.; Watanabe, Itaru; Zhu, Jing; Gottschalk, Allan; Recio-Pinto, Esperanza; Thornhill, William B.

In: Brain Research, Vol. 1058, No. 1-2, 05.10.2005, p. 30-43.

Research output: Contribution to journalArticle

Sutachan, Jhon J. ; Watanabe, Itaru ; Zhu, Jing ; Gottschalk, Allan ; Recio-Pinto, Esperanza ; Thornhill, William B. / Effects of Kv1.1 channel glycosylation on C-type inactivation and simulated action potentials. In: Brain Research. 2005 ; Vol. 1058, No. 1-2. pp. 30-43.
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AB - Kv1.1 channels are brain glycoproteins that play an important role in repolarization of action potentials. In previous work, we showed that lack of N-glycosylation, particularly lack of sialylation, of Kv1.1 affected its macroscopic gating properties and slowed activation and C-type inactivation kinetics and produced a depolarized shift in the steady-state activation curve. In our current study, we used single channel analysis to investigate voltage-independent C-type inactivation in both Kv1.1 and Kv1.1N207Q, a glycosylation mutant. Both channels underwent brief and long-lived closures, and the lifetime and frequency of the long-lived closed states were voltage-independent and similar for both channels. We found that, as in macroscopic measurements, Kv1.1N207Q exhibited a ∼8 mV positive shift in its single channel fractional open time (fo) and a shallower fo-voltage slope compared with Kv1.1. Data suggested that C-type inactivation reflected the equilibration time with at least two slow voltage-independent long-lived closed states that followed the rapid activation process. In addition, data simulation indicated that the C-type inactivation process reflected the equilibration time between the open state and at least two long-lived closed states. Moreover, the faster macroscopic current decay in Kv1.1 mostly reflected a slower equilibration time in these channels as compared with Kv1.1N207Q. Finally, action potential simulations indicated that the N207Q mutation broaden the action potential and decreased the interspike interval. The shape of the action potential was not significantly affected by C-type inactivation, however, for a given channel, C-type inactivation increased the interspike interval. Data and simulations suggested that excitable cells could use differences in K + channel glycosylation degree as an additional mechanism to increase channel functional diversity which could modify cell excitability.

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