Effects of isolated GH deficiency on adipose tissue, feeding and adipokines in mice

Lucia Recinella, Rugia Shohreh, Roberto Salvatori, Giustino Orlando, Michele Vacca, Luigi Brunetti

Research output: Contribution to journalArticle

Abstract

Objective: Growth hormone deficiency (GHD) leads to growth failure and changes in body composition including increased fat accumulation and reduced lean body mass in both humans and rodents. The aim of this study was to characterize the consequences of isolated GHD (IGHD) on adiposity, total body weight (TBW), and food intake in a mouse model of autosomal recessive IGHD due to targeted ablation of the GH-releasing hormone (GHRH) gene [GHRH knockout (GHRHKO)]. Animals were also analyzed with respect to leptin, adiponectin and visfatin circulating levels and gene expression in both intra-abdominal and subcutaneous fat. Design: We studied 8 male mice homozygous for GHRHKO allele (-/-) and 8 heterozygous (+/-) animals as controls. Feeding and TBW data were collected weekly from 3 through 5. months of age. Animals were then euthanized for measurement of body length and intra-abdominal (epididymal and retroperitoneal) and subcutaneous (interscapular, axillary, gluteal and inguinal) fat weights, and for blood collection for leptin, adiponectin and visfatin measurement. Gene expression of leptin, adiponectin and visfatin in adipose tissue was evaluated by real-time reverse transcription polymerase chain reaction. Results: GHRHKO mice had significantly increased relative intra-abdominal (P

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalGrowth Hormone and IGF Research
Volume23
Issue number6
DOIs
StatePublished - Dec 2013

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Nicotinamide Phosphoribosyltransferase
Pituitary Dwarfism
Adipokines
Adiponectin
Leptin
Adipose Tissue
Fats
Body Weight
Abdominal Subcutaneous Fat
Hormones
Gene Expression
Intra-Abdominal Fat
Groin
Adiposity
Body Composition
Knockout Mice
Growth Hormone
Reverse Transcription
Rodentia
Eating

Keywords

  • Adiponectin
  • Food intake
  • Growth hormone-releasing hormone knockout
  • Intra-abdominal fat
  • Leptin
  • Subcutaneous fat
  • Visfatin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Effects of isolated GH deficiency on adipose tissue, feeding and adipokines in mice. / Recinella, Lucia; Shohreh, Rugia; Salvatori, Roberto; Orlando, Giustino; Vacca, Michele; Brunetti, Luigi.

In: Growth Hormone and IGF Research, Vol. 23, No. 6, 12.2013, p. 237-242.

Research output: Contribution to journalArticle

Recinella, Lucia ; Shohreh, Rugia ; Salvatori, Roberto ; Orlando, Giustino ; Vacca, Michele ; Brunetti, Luigi. / Effects of isolated GH deficiency on adipose tissue, feeding and adipokines in mice. In: Growth Hormone and IGF Research. 2013 ; Vol. 23, No. 6. pp. 237-242.
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