Effects of inhibition of phosphoenolpyruvate carboxykinase on ammonia production by the rat kidney

Franklyn I. Bennett, G. A. Alleyne

Research output: Contribution to journalArticlepeer-review

Abstract

Phosphoenolpyruvate carboxykinase (PEPCK) in the renal cortex shows the expected increase in activity but oxal acetate decarboxylase and malic enzyme, two other enzymes capable of providing precursors for use in the total oxidation of glutamine by the kidney, showed no change in their activity as a result of metabolic acidosis in rats. 3-Mercaptopicolinic acid, a substance which inhibits PEPCK, caused a 23% decrease in the oxidation of glutamine to CO2 by kidney cortical slices in vitro. 3-Mercaptopicolinic acid in vivo had anomalous effects on gluconeogenesis but consistently inhibited ammoniagenesis by kidneys of normal as well as acidotic rats.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalKidney and Blood Pressure Research
Volume1
Issue number3
DOIs
StatePublished - 1978
Externally publishedYes

Keywords

  • 3-Mercaptopicolinic acid
  • Acidosis
  • Ammoniagenesis
  • Gluconeogenesis
  • Kidney Phosphoenolpyruvate carboxykinase
  • Malic enzyme
  • Oxal acetate decarboxylase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Nephrology

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