Effects of inhibition of phosphoenolpyruvate carboxykinase on ammonia production by the rat kidney

Franklyn I. Bennett; G. A. Alleyne

doi:10.1159/000172656

Effects of inhibition of phosphoenolpyruvate carboxykinase on ammonia production by the rat kidney

Franklyn I. Bennett, G. A. Alleyne

Research output: Contribution to journal › Article › peer-review

Abstract

Phosphoenolpyruvate carboxykinase (PEPCK) in the renal cortex shows the expected increase in activity but oxal acetate decarboxylase and malic enzyme, two other enzymes capable of providing precursors for use in the total oxidation of glutamine by the kidney, showed no change in their activity as a result of metabolic acidosis in rats. 3-Mercaptopicolinic acid, a substance which inhibits PEPCK, caused a 23% decrease in the oxidation of glutamine to CO₂ by kidney cortical slices in vitro. 3-Mercaptopicolinic acid in vivo had anomalous effects on gluconeogenesis but consistently inhibited ammoniagenesis by kidneys of normal as well as acidotic rats.

Original language	English (US)
Pages (from-to)	147-153
Number of pages	7
Journal	Kidney and Blood Pressure Research
Volume	1
Issue number	3
DOIs	https://doi.org/10.1159/000172656
State	Published - 1978
Externally published	Yes

Keywords

3-Mercaptopicolinic acid
Acidosis
Ammoniagenesis
Gluconeogenesis
Kidney Phosphoenolpyruvate carboxykinase
Malic enzyme
Oxal acetate decarboxylase

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine
Nephrology

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10.1159/000172656

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title = "Effects of inhibition of phosphoenolpyruvate carboxykinase on ammonia production by the rat kidney",

abstract = "Phosphoenolpyruvate carboxykinase (PEPCK) in the renal cortex shows the expected increase in activity but oxal acetate decarboxylase and malic enzyme, two other enzymes capable of providing precursors for use in the total oxidation of glutamine by the kidney, showed no change in their activity as a result of metabolic acidosis in rats. 3-Mercaptopicolinic acid, a substance which inhibits PEPCK, caused a 23% decrease in the oxidation of glutamine to CO2 by kidney cortical slices in vitro. 3-Mercaptopicolinic acid in vivo had anomalous effects on gluconeogenesis but consistently inhibited ammoniagenesis by kidneys of normal as well as acidotic rats.",

keywords = "3-Mercaptopicolinic acid, Acidosis, Ammoniagenesis, Gluconeogenesis, Kidney Phosphoenolpyruvate carboxykinase, Malic enzyme, Oxal acetate decarboxylase",

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year = "1978",

doi = "10.1159/000172656",

language = "English (US)",

volume = "1",

pages = "147--153",

journal = "Kidney and Blood Pressure Research",

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T1 - Effects of inhibition of phosphoenolpyruvate carboxykinase on ammonia production by the rat kidney

AU - Bennett, Franklyn I.

AU - Alleyne, G. A.

PY - 1978

Y1 - 1978

N2 - Phosphoenolpyruvate carboxykinase (PEPCK) in the renal cortex shows the expected increase in activity but oxal acetate decarboxylase and malic enzyme, two other enzymes capable of providing precursors for use in the total oxidation of glutamine by the kidney, showed no change in their activity as a result of metabolic acidosis in rats. 3-Mercaptopicolinic acid, a substance which inhibits PEPCK, caused a 23% decrease in the oxidation of glutamine to CO2 by kidney cortical slices in vitro. 3-Mercaptopicolinic acid in vivo had anomalous effects on gluconeogenesis but consistently inhibited ammoniagenesis by kidneys of normal as well as acidotic rats.

AB - Phosphoenolpyruvate carboxykinase (PEPCK) in the renal cortex shows the expected increase in activity but oxal acetate decarboxylase and malic enzyme, two other enzymes capable of providing precursors for use in the total oxidation of glutamine by the kidney, showed no change in their activity as a result of metabolic acidosis in rats. 3-Mercaptopicolinic acid, a substance which inhibits PEPCK, caused a 23% decrease in the oxidation of glutamine to CO2 by kidney cortical slices in vitro. 3-Mercaptopicolinic acid in vivo had anomalous effects on gluconeogenesis but consistently inhibited ammoniagenesis by kidneys of normal as well as acidotic rats.

KW - 3-Mercaptopicolinic acid

KW - Acidosis

KW - Ammoniagenesis

KW - Gluconeogenesis

KW - Kidney Phosphoenolpyruvate carboxykinase

KW - Malic enzyme

KW - Oxal acetate decarboxylase

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Effects of inhibition of phosphoenolpyruvate carboxykinase on ammonia production by the rat kidney

Abstract

Keywords

ASJC Scopus subject areas

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