Effects of in vitro exposure of alcohol on surface receptor expression of human platelets

Marcus E. McKenzie, Christopher R. Bell, Eric D. Horowitz, Benjamin R. Oshrine, Dan Atar, Victor L. Serebruany

Research output: Contribution to journalArticle

Abstract

Platelet inhibition after moderate alcohol consumption in patients with ischaemic heart disease may contribute to reducing the risk for developing acute coronary syndromes. However, the mechanism by which ethanol affects platelets is not clarified. We sought to determine the in vitro effects of alcohol on the surface expression of human platelet receptors using whole blood flow cytometry. Blood samples from 10 healthy volunteers were incubated for 30 min with 25 and 50 mmol l-1 of phosphate buffered saline diluted grain ethanol, concentrations often used in in vitro studies. The surface expression of platelet receptors was determined by flow cytometry after fixation with 2% paraformaldehyde using the following monoclonal antibodies: CD 41 (GP IIb/IIIa), CD 42b (GP Ib), CD 62p (P-selectin), CD 51/CD 61 (vitronectin receptor), CD 31 (PECAM-1), CD 107a (LAMP-1), CD 107b (LAMP-2), CD 63 (LIMP, LAMP-3) and CD 151 (PETA-3). Dose-dependent inhibition of GP IIb/IIIa, P-selectin, CD 63 and CD 107a receptor expression was observed in the ethanol-treated whole blood samples. This study for the first time establishes a direct effect of ethanol on selective major platelet receptors. Beneficial cardiovascular properties of moderate alcohol consumption may be explained by ethanol's antiplatelet action.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalClinical Physiology and Functional Imaging
Volume22
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Ethanol
Blood Platelets
Alcohols
Platelet Membrane Glycoprotein IIb
P-Selectin
Alcohol Drinking
Flow Cytometry
Integrin alphaVbeta3
CD31 Antigens
Acute Coronary Syndrome
Myocardial Ischemia
Healthy Volunteers
Phosphates
Monoclonal Antibodies
In Vitro Techniques
Inhibition (Psychology)

Keywords

  • Alcohol
  • Coronary heart disease
  • Flow cytometry
  • Human
  • Platelets

ASJC Scopus subject areas

  • Physiology

Cite this

McKenzie, M. E., Bell, C. R., Horowitz, E. D., Oshrine, B. R., Atar, D., & Serebruany, V. L. (2002). Effects of in vitro exposure of alcohol on surface receptor expression of human platelets. Clinical Physiology and Functional Imaging, 22(2), 153-156. https://doi.org/10.1046/j.1365-2281.2002.00411.x

Effects of in vitro exposure of alcohol on surface receptor expression of human platelets. / McKenzie, Marcus E.; Bell, Christopher R.; Horowitz, Eric D.; Oshrine, Benjamin R.; Atar, Dan; Serebruany, Victor L.

In: Clinical Physiology and Functional Imaging, Vol. 22, No. 2, 2002, p. 153-156.

Research output: Contribution to journalArticle

McKenzie, Marcus E. ; Bell, Christopher R. ; Horowitz, Eric D. ; Oshrine, Benjamin R. ; Atar, Dan ; Serebruany, Victor L. / Effects of in vitro exposure of alcohol on surface receptor expression of human platelets. In: Clinical Physiology and Functional Imaging. 2002 ; Vol. 22, No. 2. pp. 153-156.
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