Effects of hypoxia in porcine pulmonary arterial myocytes: Roles of K(v) channel and endothelin-1

James Sham, Benjamin R. Crenshaw, Li Hua Deng, Larissa Shimoda, J. T. Sylvester

Research output: Contribution to journalArticle

Abstract

Effects of acute hypoxia on intracellular Ca2+ concentration ([Ca2+](i)) and cell length were recorded simultaneously in proximal and distal pulmonary (PASMCs) and femoral (FASMCs) arterial smooth muscle cells. Reducing Po2 from normoxia to severe hypoxia (Po2 <10 mmHg) caused small but significant decreases in length and a reversible increase in [Ca2+](i) in distal PASMCs and a small decrease in length in proximal PASMCs but had no effect in FASMCs, even though all three cell types contracted significantly to vasoactive agonists. Inhibition of voltage-dependent K+ (K(v)) channel with 4-aminopyridine produced a greater increase in [Ca2+](i) in distal than in proximal PASMCs. In distal PASMCs, severe hypoxia caused a slight inhibition of K(v) currents; however, it elicited further contraction in the presence of 4-aminopyridine. Endothelin-1 (10-10 M), which itself did not alter cell length or [Ca2+](i), significantly potentiated the hypoxic contraction. These results suggest that hypoxia only has small direct effects on porcine PASMCs. These effects cannot be fully explained by inhibition of K(v) channels and were greatly enhanced via synergistic interactions with the endothelium-derived factor endothelin-1.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume279
Issue number2 23-2
StatePublished - 2000

Fingerprint

Endothelin-1
Muscle Cells
Swine
Lung
4-Aminopyridine
Vasoconstrictor Agents
Thigh
Smooth Muscle Myocytes
Endothelium
Hypoxia

Keywords

  • Calcium
  • Pulmonary arterial smooth muscle cell
  • Vasoconstriction
  • Voltage-dependent potassium channel

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Effects of hypoxia in porcine pulmonary arterial myocytes : Roles of K(v) channel and endothelin-1. / Sham, James; Crenshaw, Benjamin R.; Deng, Li Hua; Shimoda, Larissa; Sylvester, J. T.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 279, No. 2 23-2, 2000.

Research output: Contribution to journalArticle

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