Effects of glucosinolate-rich broccoli sprouts on urinary levels of aflatoxin-DNA adducts and phenanthrene tetraols in a randomized clinical trial in He Zuo Township, Qidong, People's Republic of China

Thomas W Kensler, Jian Guo Chen, Patricia Egner, Jed W Fahey, Lisa Paula Jacobson, Katherine K. Stephenson, Lingxiang Ye, Jamie L. Coady, Jin Bing Wang, Yan Wu, Yan Sun, Qi Nan Zhang, Bao Chu Zhang, Yuan Rong Zhu, Geng Sun Qian, Stephen G. Carmella, Stephen S. Hecht, Lorie Benning, Stephen J Gange, John Davis Groopman & 1 others Paul Talalay

Research output: Contribution to journalArticle

Abstract

Residents of Qidong, People's Republic of China, are at high risk for development of hepatocellular carcinoma, in part due to consumption of aflatoxin-contaminated foods, and are exposed to high levels of phenanthrene, a sentinel of hydrocarbon air toxics. Cruciferous vegetables, such as broccoli, contain anticarcinogens. Glucoraphanin, the principal glucosinolate in broccoli sprouts, can be hydrolyzed by gut microflora to sulforaphane, a potent inducer of carcinogen detoxication enzymes. In a randomized, placebo-controlled chemoprevention trial, we tested whether drinking hot water infusions of 3-day-old broccoli sprouts, containing defined concentrations of glucosinolates, could alter the disposition of aflatoxin and phenanthrene. Two hundred healthy adults drank infusions containing either 400 or 7-guanine were not different between the two intervention arms (P = 0.68). However, measurement of urinary levels of dithiocarbamates (sulforaphane metabolites) indicated striking interindividual differences in bioavailability. An inverse association was observed for excretion of dithiocarbamates and aflatoxin-DNA adducts (P = 0.002; R = 0.31) in individuals receiving broccoli sprout glucosinolates. Moreover, trans, anti-phenanthrene tetraol, a metabolite of the combustion product phenanthrene, was detected in urine of all participants and showed a robust inverse association with dithiocarbamate levels (P = 0.0001; R = 0.39), although again no overall difference between intervention arms was observed (P = 0.29). Understanding factors influencing glucosinolate hydrolysis and bioavailability will be required for optimal use of broccoli sprouts in human interventions.

Original languageEnglish (US)
Pages (from-to)2605-2613
Number of pages9
JournalCancer Epidemiology Biomarkers and Prevention
Volume14
Issue number11 I
DOIs
StatePublished - Nov 2005

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Glucosinolates
Aflatoxins
DNA Adducts
Brassica
China
Randomized Controlled Trials
Biological Availability
Anticarcinogenic Agents
Poisons
Chemoprevention
Guanine
Hydrocarbons
Drinking Water
Vegetables
Carcinogens
Hepatocellular Carcinoma
Hydrolysis
Air
Placebos
phenanthrene tetraol

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Effects of glucosinolate-rich broccoli sprouts on urinary levels of aflatoxin-DNA adducts and phenanthrene tetraols in a randomized clinical trial in He Zuo Township, Qidong, People's Republic of China. / Kensler, Thomas W; Chen, Jian Guo; Egner, Patricia; Fahey, Jed W; Jacobson, Lisa Paula; Stephenson, Katherine K.; Ye, Lingxiang; Coady, Jamie L.; Wang, Jin Bing; Wu, Yan; Sun, Yan; Zhang, Qi Nan; Zhang, Bao Chu; Zhu, Yuan Rong; Qian, Geng Sun; Carmella, Stephen G.; Hecht, Stephen S.; Benning, Lorie; Gange, Stephen J; Groopman, John Davis; Talalay, Paul.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 14, No. 11 I, 11.2005, p. 2605-2613.

Research output: Contribution to journalArticle

Kensler, Thomas W ; Chen, Jian Guo ; Egner, Patricia ; Fahey, Jed W ; Jacobson, Lisa Paula ; Stephenson, Katherine K. ; Ye, Lingxiang ; Coady, Jamie L. ; Wang, Jin Bing ; Wu, Yan ; Sun, Yan ; Zhang, Qi Nan ; Zhang, Bao Chu ; Zhu, Yuan Rong ; Qian, Geng Sun ; Carmella, Stephen G. ; Hecht, Stephen S. ; Benning, Lorie ; Gange, Stephen J ; Groopman, John Davis ; Talalay, Paul. / Effects of glucosinolate-rich broccoli sprouts on urinary levels of aflatoxin-DNA adducts and phenanthrene tetraols in a randomized clinical trial in He Zuo Township, Qidong, People's Republic of China. In: Cancer Epidemiology Biomarkers and Prevention. 2005 ; Vol. 14, No. 11 I. pp. 2605-2613.
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abstract = "Residents of Qidong, People's Republic of China, are at high risk for development of hepatocellular carcinoma, in part due to consumption of aflatoxin-contaminated foods, and are exposed to high levels of phenanthrene, a sentinel of hydrocarbon air toxics. Cruciferous vegetables, such as broccoli, contain anticarcinogens. Glucoraphanin, the principal glucosinolate in broccoli sprouts, can be hydrolyzed by gut microflora to sulforaphane, a potent inducer of carcinogen detoxication enzymes. In a randomized, placebo-controlled chemoprevention trial, we tested whether drinking hot water infusions of 3-day-old broccoli sprouts, containing defined concentrations of glucosinolates, could alter the disposition of aflatoxin and phenanthrene. Two hundred healthy adults drank infusions containing either 400 or 7-guanine were not different between the two intervention arms (P = 0.68). However, measurement of urinary levels of dithiocarbamates (sulforaphane metabolites) indicated striking interindividual differences in bioavailability. An inverse association was observed for excretion of dithiocarbamates and aflatoxin-DNA adducts (P = 0.002; R = 0.31) in individuals receiving broccoli sprout glucosinolates. Moreover, trans, anti-phenanthrene tetraol, a metabolite of the combustion product phenanthrene, was detected in urine of all participants and showed a robust inverse association with dithiocarbamate levels (P = 0.0001; R = 0.39), although again no overall difference between intervention arms was observed (P = 0.29). Understanding factors influencing glucosinolate hydrolysis and bioavailability will be required for optimal use of broccoli sprouts in human interventions.",
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AU - Kensler, Thomas W

AU - Chen, Jian Guo

AU - Egner, Patricia

AU - Fahey, Jed W

AU - Jacobson, Lisa Paula

AU - Stephenson, Katherine K.

AU - Ye, Lingxiang

AU - Coady, Jamie L.

AU - Wang, Jin Bing

AU - Wu, Yan

AU - Sun, Yan

AU - Zhang, Qi Nan

AU - Zhang, Bao Chu

AU - Zhu, Yuan Rong

AU - Qian, Geng Sun

AU - Carmella, Stephen G.

AU - Hecht, Stephen S.

AU - Benning, Lorie

AU - Gange, Stephen J

AU - Groopman, John Davis

AU - Talalay, Paul

PY - 2005/11

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N2 - Residents of Qidong, People's Republic of China, are at high risk for development of hepatocellular carcinoma, in part due to consumption of aflatoxin-contaminated foods, and are exposed to high levels of phenanthrene, a sentinel of hydrocarbon air toxics. Cruciferous vegetables, such as broccoli, contain anticarcinogens. Glucoraphanin, the principal glucosinolate in broccoli sprouts, can be hydrolyzed by gut microflora to sulforaphane, a potent inducer of carcinogen detoxication enzymes. In a randomized, placebo-controlled chemoprevention trial, we tested whether drinking hot water infusions of 3-day-old broccoli sprouts, containing defined concentrations of glucosinolates, could alter the disposition of aflatoxin and phenanthrene. Two hundred healthy adults drank infusions containing either 400 or 7-guanine were not different between the two intervention arms (P = 0.68). However, measurement of urinary levels of dithiocarbamates (sulforaphane metabolites) indicated striking interindividual differences in bioavailability. An inverse association was observed for excretion of dithiocarbamates and aflatoxin-DNA adducts (P = 0.002; R = 0.31) in individuals receiving broccoli sprout glucosinolates. Moreover, trans, anti-phenanthrene tetraol, a metabolite of the combustion product phenanthrene, was detected in urine of all participants and showed a robust inverse association with dithiocarbamate levels (P = 0.0001; R = 0.39), although again no overall difference between intervention arms was observed (P = 0.29). Understanding factors influencing glucosinolate hydrolysis and bioavailability will be required for optimal use of broccoli sprouts in human interventions.

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