Effects of gene transfer into keratocytes on corneal wound healing after excimer laser keratectomy

L. Moreira, D. Sanchez, E. M. Gordon, W. F. Anderson, P. J. McDonnell

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose. To determine the potential o "somatic gene transfer with the herpes simplex virus thymidine kinase (HStk) gene ;is a technique for modulating corneal wound healing after superficial keratectomy. Methods. PTK in a depth of 100 microns was performed in 8 rabbit corneas. E> pression of the 8-gaIactosidase gene was demonstrated with histochemical stair ing. Four rabbits were transduced with vector containing the Hstk gene, and a control group of four eyes was transduced with "empty" vector. Topical ganciclovir (20 mg/ml) was subsequently applied for 10 days during the postoperative period. I'ost-surgical comeal haze was measured using a standardized videographic techn que with computerized image analysis to quantitate corneal opacity. Results. Expression of β-galactosidase within vimentinpositive corneal stromal cells confirmed transduction of keratocytes in the rabbits. The combination of Hstk gene transfei and topical ganciclovir reduced postoperative corneal opacity compared to controls (P=0.03). Conclusions. The ability to genetically transduce stromal keratoc;/tes provides a new strategy for manipulating the important cellular and molecular el ents that influence corneal wound healing, thus offering a potential approach to prevent scarring after excimer keratectomy.

Original languageEnglish (US)
Pages (from-to)S401
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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