Effects of gamma-interferon on keratocyte-induced collagen gel contraction and keratocyte proliferation

Alireza Pakkar, Roya Rofougaran, Ken Lu, Won R. Wee, Amir K. Isfahani, Peter J McDonnell

Research output: Contribution to journalArticle

Abstract

PURPOSE: Gamma-interferon has been shown to be an effective immunoregulatory polypeptide that can modulate fibroblastic response. We investigated the effects of gamma-interferon on keratocyte proliferation and keratocyte-induced collagen gel contraction. METHODS: Gamma-interferon in concentrations of 0.01, 1, 100, and 1000 U/ml of media was added to keratocytes embedded in polymerized type I collagen and the gel area was measured after 5 days with an image analysis system. The rate of keratocyte proliferation within and outside the collagen gel under the influence of gamma-interferon was also investigated. RESULTS: Keratocyte-induced collagen gel contraction was significantly inhibited at all concentrations above 0.01 U/ml. The keratocyte proliferation was not affected by low and moderate concentrations and was significantly stimulated at concentration of 1000 U/ml. CONCLUSION: Keratocyte-induced collagen gel contraction is inhibited by gamma-interferon and the mechanism of this effect is not inhibition of keratocyte proliferation by gamma-interferon.

Original languageEnglish (US)
Pages (from-to)152-155
Number of pages4
JournalJournal of Refractive Surgery
Volume14
Issue number2
StatePublished - Mar 1998
Externally publishedYes

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Interferon-gamma
Collagen
Gels
Collagen Type I
Peptides

ASJC Scopus subject areas

  • Ophthalmology

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Effects of gamma-interferon on keratocyte-induced collagen gel contraction and keratocyte proliferation. / Pakkar, Alireza; Rofougaran, Roya; Lu, Ken; Wee, Won R.; Isfahani, Amir K.; McDonnell, Peter J.

In: Journal of Refractive Surgery, Vol. 14, No. 2, 03.1998, p. 152-155.

Research output: Contribution to journalArticle

Pakkar, Alireza ; Rofougaran, Roya ; Lu, Ken ; Wee, Won R. ; Isfahani, Amir K. ; McDonnell, Peter J. / Effects of gamma-interferon on keratocyte-induced collagen gel contraction and keratocyte proliferation. In: Journal of Refractive Surgery. 1998 ; Vol. 14, No. 2. pp. 152-155.
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AU - Pakkar, Alireza

AU - Rofougaran, Roya

AU - Lu, Ken

AU - Wee, Won R.

AU - Isfahani, Amir K.

AU - McDonnell, Peter J

PY - 1998/3

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N2 - PURPOSE: Gamma-interferon has been shown to be an effective immunoregulatory polypeptide that can modulate fibroblastic response. We investigated the effects of gamma-interferon on keratocyte proliferation and keratocyte-induced collagen gel contraction. METHODS: Gamma-interferon in concentrations of 0.01, 1, 100, and 1000 U/ml of media was added to keratocytes embedded in polymerized type I collagen and the gel area was measured after 5 days with an image analysis system. The rate of keratocyte proliferation within and outside the collagen gel under the influence of gamma-interferon was also investigated. RESULTS: Keratocyte-induced collagen gel contraction was significantly inhibited at all concentrations above 0.01 U/ml. The keratocyte proliferation was not affected by low and moderate concentrations and was significantly stimulated at concentration of 1000 U/ml. CONCLUSION: Keratocyte-induced collagen gel contraction is inhibited by gamma-interferon and the mechanism of this effect is not inhibition of keratocyte proliferation by gamma-interferon.

AB - PURPOSE: Gamma-interferon has been shown to be an effective immunoregulatory polypeptide that can modulate fibroblastic response. We investigated the effects of gamma-interferon on keratocyte proliferation and keratocyte-induced collagen gel contraction. METHODS: Gamma-interferon in concentrations of 0.01, 1, 100, and 1000 U/ml of media was added to keratocytes embedded in polymerized type I collagen and the gel area was measured after 5 days with an image analysis system. The rate of keratocyte proliferation within and outside the collagen gel under the influence of gamma-interferon was also investigated. RESULTS: Keratocyte-induced collagen gel contraction was significantly inhibited at all concentrations above 0.01 U/ml. The keratocyte proliferation was not affected by low and moderate concentrations and was significantly stimulated at concentration of 1000 U/ml. CONCLUSION: Keratocyte-induced collagen gel contraction is inhibited by gamma-interferon and the mechanism of this effect is not inhibition of keratocyte proliferation by gamma-interferon.

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