Effects of galanin on short circuit current and electrolyte transport in rabbit ileum

Fadia R. Homaidan, Shao Hua Tang, Mark Donowitz, Geoffrey W.G. Sharp

Research output: Contribution to journalArticle

Abstract

Galanin decreased short circuit current (Isc) and increased. Galanin decreased short circuit current (Isc) and increased active Na+ and Cl- absorption in rabbit ileum. In the absence of calcium, the galanin-induced decrease in Isc was inhibited by approximately 60%. Tetrodotoxin significantly reduced the effect of galanin on Isc, and tetrodotoxin and EGTA totally blocked the effect, indicating that the nonneuronal mediator of the effect is Ca2+ dependent. Galanin binding to basolateral membranes prepared from ileal epithelial cells was specific and of high affinity. These results suggest the involvement of this peptide in the regulation of intestinal epithelial cell function.

Original languageEnglish (US)
Pages (from-to)1431-1436
Number of pages6
JournalPeptides
Volume15
Issue number8
DOIs
StatePublished - 1994

Keywords

  • Electrolyte transport
  • Galanin
  • NaCl absorption

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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