Effects of galanin on short circuit current and electrolyte transport in rabbit ileum

Fadia R. Homaidan; Shao Hua Tang; Mark Donowitz; Geoffrey W.G. Sharp

doi:10.1016/0196-9781(94)90120-1

Effects of galanin on short circuit current and electrolyte transport in rabbit ileum

Fadia R. Homaidan, Shao Hua Tang, Mark Donowitz, Geoffrey W.G. Sharp

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Galanin decreased short circuit current (I_sc) and increased. Galanin decreased short circuit current (I_sc) and increased active Na⁺ and Cl^- absorption in rabbit ileum. In the absence of calcium, the galanin-induced decrease in I_sc was inhibited by approximately 60%. Tetrodotoxin significantly reduced the effect of galanin on I_sc, and tetrodotoxin and EGTA totally blocked the effect, indicating that the nonneuronal mediator of the effect is Ca²⁺ dependent. Galanin binding to basolateral membranes prepared from ileal epithelial cells was specific and of high affinity. These results suggest the involvement of this peptide in the regulation of intestinal epithelial cell function.

Original language	English (US)
Pages (from-to)	1431-1436
Number of pages	6
Journal	Peptides
Volume	15
Issue number	8
DOIs	https://doi.org/10.1016/0196-9781(94)90120-1
State	Published - 1994
Externally published	Yes

Keywords

Electrolyte transport
Galanin
NaCl absorption

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(94)90120-1

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title = "Effects of galanin on short circuit current and electrolyte transport in rabbit ileum",

abstract = "Galanin decreased short circuit current (Isc) and increased. Galanin decreased short circuit current (Isc) and increased active Na+ and Cl- absorption in rabbit ileum. In the absence of calcium, the galanin-induced decrease in Isc was inhibited by approximately 60%. Tetrodotoxin significantly reduced the effect of galanin on Isc, and tetrodotoxin and EGTA totally blocked the effect, indicating that the nonneuronal mediator of the effect is Ca2+ dependent. Galanin binding to basolateral membranes prepared from ileal epithelial cells was specific and of high affinity. These results suggest the involvement of this peptide in the regulation of intestinal epithelial cell function.",

keywords = "Electrolyte transport, Galanin, NaCl absorption",

author = "Homaidan, {Fadia R.} and Tang, {Shao Hua} and Mark Donowitz and Sharp, {Geoffrey W.G.}",

year = "1994",

doi = "10.1016/0196-9781(94)90120-1",

language = "English (US)",

volume = "15",

pages = "1431--1436",

journal = "Peptides",

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TY - JOUR

T1 - Effects of galanin on short circuit current and electrolyte transport in rabbit ileum

AU - Homaidan, Fadia R.

AU - Tang, Shao Hua

AU - Donowitz, Mark

AU - Sharp, Geoffrey W.G.

PY - 1994

Y1 - 1994

N2 - Galanin decreased short circuit current (Isc) and increased. Galanin decreased short circuit current (Isc) and increased active Na+ and Cl- absorption in rabbit ileum. In the absence of calcium, the galanin-induced decrease in Isc was inhibited by approximately 60%. Tetrodotoxin significantly reduced the effect of galanin on Isc, and tetrodotoxin and EGTA totally blocked the effect, indicating that the nonneuronal mediator of the effect is Ca2+ dependent. Galanin binding to basolateral membranes prepared from ileal epithelial cells was specific and of high affinity. These results suggest the involvement of this peptide in the regulation of intestinal epithelial cell function.

AB - Galanin decreased short circuit current (Isc) and increased. Galanin decreased short circuit current (Isc) and increased active Na+ and Cl- absorption in rabbit ileum. In the absence of calcium, the galanin-induced decrease in Isc was inhibited by approximately 60%. Tetrodotoxin significantly reduced the effect of galanin on Isc, and tetrodotoxin and EGTA totally blocked the effect, indicating that the nonneuronal mediator of the effect is Ca2+ dependent. Galanin binding to basolateral membranes prepared from ileal epithelial cells was specific and of high affinity. These results suggest the involvement of this peptide in the regulation of intestinal epithelial cell function.

KW - Electrolyte transport

KW - Galanin

KW - NaCl absorption

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ER -

Effects of galanin on short circuit current and electrolyte transport in rabbit ileum

Abstract

Keywords

ASJC Scopus subject areas

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