Effects of fatty acid amide hydrolase inhibition on neuronal responses to nicotine, cocaine and morphine in the nucleus accumbens shell and ventral tegmental area: Involvement of PPAR-α nuclear receptors

Antonio Luchicchi, Salvatore Lecca, Stefano Carta, Giuliano Pillolla, Anna L. Muntoni, Sevil Yasar, Steven R. Goldberg, Marco Pistis

Research output: Contribution to journalArticle

Abstract

The endocannabinoid system regulates neurotransmission in brain regions relevant to neurobiological and behavioral actions of addicting drugs. We recently demonstrated that inhibition by URB597 of fatty acid amide hydrolase (FAAH), the main enzyme that degrades the endogenous cannabinoid N-acylethanolamine (NAE) anandamide and the endogenous non-cannabinoid NAEs oleoylethanolamide and palmitoylethanolamide, blocks nicotine-induced excitation of ventral tegmental area (VTA) dopamine (DA) neurons and DA release in the shell of the nucleus accumbens (ShNAc), as well as nicotine-induced drug self-administration, conditioned place preference and relapse in rats. Here, we studied whether effects of FAAH inhibition on nicotine-induced changes in activity of VTA DA neurons were specific for nicotine or extended to two drugs of abuse acting through different mechanisms, cocaine and morphine. We also evaluated whether FAAH inhibition affects nicotine-, cocaine- or morphine-induced actions in the ShNAc. Experiments involved single-unit electrophysiological recordings from DA neurons in the VTA and medium spiny neurons in the ShNAc in anesthetized rats. We found that URB597 blocked effects of nicotine and cocaine in the ShNAc through activation of both surface cannabinoid CB1-receptors and alpha-type peroxisome proliferator-activated nuclear receptor. URB597 did not alter the effects of either cocaine or morphine on VTA DA neurons. These results show that the blockade of nicotine-induced excitation of VTA DA neurons, which we previously described, is selective for nicotine and indicate novel mechanisms recruited to regulate the effects of addicting drugs within the ShNAc of the brain reward system.

Original languageEnglish (US)
Pages (from-to)277-288
Number of pages12
JournalAddiction Biology
Volume15
Issue number3
DOIs
StatePublished - 2010

Fingerprint

Ventral Tegmental Area
Peroxisome Proliferator-Activated Receptors
Nucleus Accumbens
Cytoplasmic and Nuclear Receptors
Nicotine
Cocaine
Morphine
Dopaminergic Neurons
Pharmaceutical Preparations
Cannabinoid Receptor CB1
Endocannabinoids
Self Administration
Cannabinoids
Inhibition (Psychology)
fatty-acid amide hydrolase
Brain
Street Drugs
Reward
Synaptic Transmission
Dopamine

Keywords

  • Cocaine
  • Dopamine neurons
  • Electrophysiology
  • Nicotine
  • Nucleus accumbens
  • Peroxisome proliferator-activated receptor-α

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Pharmacology
  • Medicine(all)

Cite this

Effects of fatty acid amide hydrolase inhibition on neuronal responses to nicotine, cocaine and morphine in the nucleus accumbens shell and ventral tegmental area : Involvement of PPAR-α nuclear receptors. / Luchicchi, Antonio; Lecca, Salvatore; Carta, Stefano; Pillolla, Giuliano; Muntoni, Anna L.; Yasar, Sevil; Goldberg, Steven R.; Pistis, Marco.

In: Addiction Biology, Vol. 15, No. 3, 2010, p. 277-288.

Research output: Contribution to journalArticle

Luchicchi, Antonio ; Lecca, Salvatore ; Carta, Stefano ; Pillolla, Giuliano ; Muntoni, Anna L. ; Yasar, Sevil ; Goldberg, Steven R. ; Pistis, Marco. / Effects of fatty acid amide hydrolase inhibition on neuronal responses to nicotine, cocaine and morphine in the nucleus accumbens shell and ventral tegmental area : Involvement of PPAR-α nuclear receptors. In: Addiction Biology. 2010 ; Vol. 15, No. 3. pp. 277-288.
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AU - Lecca, Salvatore

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