TY - JOUR
T1 - Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice
AU - Xia, Lu
AU - Yuan, Yao Zong
AU - Xu, Chun Di
AU - Zhang, Yong Pin
AU - Qiao, Ming Ming
AU - Xu, Jia Xu
PY - 2002
Y1 - 2002
N2 - Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice. Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5 mm3) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 μg.kg-1) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice. Results: Within the concentration range of 0.05-100 mg.L-1, rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100% vs 102.8%, P < 0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 μg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P > 0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P > 0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g (P > 0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth. Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.
AB - Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice. Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5 mm3) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 μg.kg-1) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice. Results: Within the concentration range of 0.05-100 mg.L-1, rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100% vs 102.8%, P < 0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 μg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P > 0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P > 0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g (P > 0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth. Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0036283347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036283347&partnerID=8YFLogxK
U2 - 10.3748/wjg.v8.i3.455
DO - 10.3748/wjg.v8.i3.455
M3 - Article
C2 - 12046069
AN - SCOPUS:0036283347
SN - 1007-9327
VL - 8
SP - 455
EP - 458
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 3
ER -