Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice

Lu Xia, Yao Zong Yuan, Chun Di Xu, Yong Pin Zhang, Ming Ming Qiao, Jia Xu Xu

Research output: Contribution to journalArticle

Abstract

Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice. Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5 mm3) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 μg.kg-1) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice. Results: Within the concentration range of 0.05-100 mg.L-1, rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100% vs 102.8%, P <0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 μg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P > 0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P > 0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g (P > 0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth. Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.

Original languageEnglish (US)
Pages (from-to)455-458
Number of pages4
JournalWorld Journal of Gastroenterology
Volume8
Issue number3
StatePublished - 2002
Externally publishedYes

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Epidermal Growth Factor
Nude Mice
Stomach Neoplasms
Growth
Neoplasms
Stomach
Epithelial Cells
3T3 Cells
Cytoprotection
Weight-Bearing
Stomach Ulcer
Growth and Development
Cell Differentiation
Adenocarcinoma
Body Weight
Cell Line

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Xia, L., Yuan, Y. Z., Xu, C. D., Zhang, Y. P., Qiao, M. M., & Xu, J. X. (2002). Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice. World Journal of Gastroenterology, 8(3), 455-458.

Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice. / Xia, Lu; Yuan, Yao Zong; Xu, Chun Di; Zhang, Yong Pin; Qiao, Ming Ming; Xu, Jia Xu.

In: World Journal of Gastroenterology, Vol. 8, No. 3, 2002, p. 455-458.

Research output: Contribution to journalArticle

Xia, Lu ; Yuan, Yao Zong ; Xu, Chun Di ; Zhang, Yong Pin ; Qiao, Ming Ming ; Xu, Jia Xu. / Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice. In: World Journal of Gastroenterology. 2002 ; Vol. 8, No. 3. pp. 455-458.
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title = "Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice",
abstract = "Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice. Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5 mm3) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 μg.kg-1) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice. Results: Within the concentration range of 0.05-100 mg.L-1, rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100{\%} vs 102.8{\%}, P <0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 μg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P > 0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P > 0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g (P > 0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth. Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.",
author = "Lu Xia and Yuan, {Yao Zong} and Xu, {Chun Di} and Zhang, {Yong Pin} and Qiao, {Ming Ming} and Xu, {Jia Xu}",
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T1 - Effects of epidermal growth factor on the growth of human gastric cancer cell and the implanted tumor of nude mice

AU - Xia, Lu

AU - Yuan, Yao Zong

AU - Xu, Chun Di

AU - Zhang, Yong Pin

AU - Qiao, Ming Ming

AU - Xu, Jia Xu

PY - 2002

Y1 - 2002

N2 - Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice. Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5 mm3) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 μg.kg-1) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice. Results: Within the concentration range of 0.05-100 mg.L-1, rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100% vs 102.8%, P <0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 μg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P > 0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P > 0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g (P > 0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth. Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.

AB - Aim: Epidermal growth factor (EGF) plays an important role in the regulation of gastrointestinal tissue growth and development, and it can stimulate epithelial proliferation, cell differentiation and growth. It has been established that the EGF can promote gastric cytoprotection and ulcer healing. But the potential ability of EGF to regulate the gastric cancer growth is unknown. This study is to investigate the influence of EGF on human gastric cancer cell and the implanted tumor growth of nude mice. Methods: The cell growth rates of human gastric adenocarcinoma cell lines MKN-28, MKN-45, SGC-7901 and normal human gastric epithelial cells 3T3 were assessed when incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method. The cells of MKN-28, MKN-45, SGC-7901 (gastric cancer tissue 1.5 mm3) were implanted in the BALB/cA nude mice for 10 days. The EGF was given intraperitoneally (15, 30, 60 μg.kg-1) for 3 weeks. The body weights of the tumor-bearing animals and their tumor mass were measured afterwards to assess the mitogenic effect of rhEGF in the nude mice. Results: Within the concentration range of 0.05-100 mg.L-1, rhEGF could increase the cell growth of normal 3T3 cells (cell growth rate 100% vs 102.8%, P <0.05), but partially restrain the gastric cancer cell growth. The latter effect was related to cell differentiation. In 15-60 μg/kg rhEGF groups, the mean implanted tumor mass of MKN-28 cell were 1.75 g, 1.91 g, 2.08 g/NS group 1.97 g (P > 0.05), the mean tumor mass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group 1.07 g (P > 0.05), and for MKN-45 cell, the tumor mass were respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g (P > 0.05). So rhEGF had no obvious effect on implanted MKN-28, SGC-7901 and MKN-45 tumor growth. Conclusion: EGF has no stimulating effect on the human gastric cancer cell growth neither in vitro nor in vivo.

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